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| 山羊痘病毒非必需基因区gp024基因的鉴定 | |
| 引用本文： | 雷震,王芳,马文戈,黄炯,符子华,于力,冉多良. 山羊痘病毒非必需基因区gp024基因的鉴定[J]. 中国预防兽医学报, 2009, 31(11) |
| 作者姓名： | 雷震 王芳 马文戈 黄炯 符子华 于力 冉多良 |
| 作者单位： | 1. 新疆畜牧科学院,兽医研究所,新疆,乌鲁木齐,830000;新疆农业大学,新疆,乌鲁木齐,830000 2. 中国农业科学院,哈尔滨兽医研究所,黑龙江,哈尔滨,150001 3. 新疆畜牧科学院,兽医研究所,新疆,乌鲁木齐,830000 4. 新疆农业大学,新疆,乌鲁木齐,830000 |
| 摘 要： | 为筛选山羊痘病毒(GTPV)的外源基因插入区,本研究以GTPV Pellor株为模板,设计2对引物,PCR扩增AV 41株GTPV_gp024基因相邻上下游长度约1.1 kb的同源重组片段,分别插入质粒pGPT-EGFP中.插入部位在痘病毒双向启动子p11～p7.5启动的报告基因EGFP-GPT上下游,构建转移载体pEG024,并转染已感染GTPV Av 41病毒的LT细胞,经GPT加压筛选7代后得到重组病毒.结果显示,重组病毒稳定表达报告基因EGFP,从而表明GTPV_gp024基因能够作为外源基因的插入区. |
| 关 键 词： | 山羊痘病毒 GTPV_gp024基因座 重组病毒 |
Identification of non-essential gp024 gene in Goatpox virus |
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| LEI Zhen,WANG Fang,MA Wen-ge,HUANG Jiong,FU Zi-hua,YU Li,RAN Duo-liang. Identification of non-essential gp024 gene in Goatpox virus[J]. Chinese Journal of Preventive Veterinary Medicine, 2009, 31(11) | |
| Authors: | LEI Zhen WANG Fang MA Wen-ge HUANG Jiong FU Zi-hua YU Li RAN Duo-liang |
| Abstract: | In this study, two circumferential fragments covering 1.1 kb approximately of the locus of "GTPV_gp024" were amplified by PCR with specific primers based on GTPV strain Pellor. The PCR fragments were cloned into upstream and downstream of the enhanced green fluorescent protein (EGFP) of pGPT-EGFP vector modulated by bi-directional promoter pl 1-p7.5 originated from fowlpox virus. The recombinant transfer vector was co-transinfected into GTPV strain AV 41 infected LT cells. A recombinant GTPV strain was obtained after 7 rounds screening under GPT pressure. The results showed that EGFP was expressed stably by the recombinant GTPV, and the GTPV_gp024 locus could be used as a novel region for expression of foreign genes. |
| Keywords: | Goatpox virus locus " GTPV_gp024" recombinant virus |
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