| 江西柑橘地方品种资源及野生近缘种SSR分子标记 |
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| 引用本文： | 刘勇,吴波,刘德春,孙中海. 江西柑橘地方品种资源及野生近缘种SSR分子标记[J]. 江西农业大学学报, 2005, 27(4): 486-490 |
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| 作者姓名： | 刘勇 吴波 刘德春 孙中海 |
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| 作者单位： | 华中农业大学柑桔研究所,湖北武汉,430070;江西农业大学农学院,江西南昌,330045;江西农业大学农学院,江西南昌,330045;华中农业大学柑桔研究所,湖北武汉,430070 |
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| 基金项目： | 国家自然科学基金资助项目（30070528）和教育部优秀青年教师基金资助 |
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| 摘 要： | 利用SSR分子标记方法研究了63个江西柑橘地方品种资源及野生近缘种的遗传多样性，用30对SSR引物，检测到374条等位基因，平均每对引物检测到等位基因3～28个，平均12．47个，平均信息量为0．805。用UPGMA方法可将63个试材划分为8个类群．聚类结果可以推断出崇义野生橘可能是江西柑橘的始祖，井冈野生橙可能是橘类和橙类的杂交种。
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| 关 键 词： | 江西柑橘 种质资源 遗传多样性 SSR分子标记 UPGMA聚类分析 |
| 文章编号： | 1000-2286（2005）04-0486-05 |
| 收稿时间： | 2005-02-28 |
| 修稿时间： | 2005-02-28 |
On Genetic Diversity of Jiangxi Native Citrus and its Wild Varieties Based on SSR Markers |
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| LIU Yong,WU Bo,LIU De-chun,SUN Zhong-hai. On Genetic Diversity of Jiangxi Native Citrus and its Wild Varieties Based on SSR Markers[J]. Acta Agriculturae Universitis Jiangxiensis, 2005, 27(4): 486-490 |
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| Authors: | LIU Yong WU Bo LIU De-chun SUN Zhong-hai |
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| Abstract: | The genetic diversity of 63 Jiangxi native citrus varieties and its wild varieties was analyzed based on microsatellite (SSR) markers. With 30 pairs of SSR primers, totally 374 polymorphic bonds were produced. The average number of alleles per SSR locus was 12.47 with a range from 3 to 28. The value of polymorphism information content (PIC) was on an average of 0.805 for each SSR locus. The 63 accession of Jiangxi native citrus varieties and its wild varieties could be divided into eight group with UPGMA method at a similarity coefficient of 0.51. It could be speculated that the original of Jiangxi citrus came from Citrus reticulate cv. Chongyi yeshengju, and Jianggang yesheng cheng was speculated the hybrid of Tangerine and orange. |
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| Keywords: | Jiangxi citrus germplasm resource genetic diversity SSR markers UPGMA cluster analysis |
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