Protamex~酶解玉米蛋白质制备生物活性玉米肽的研究

利用Protamex~蛋白酶控制玉米蛋白水解,制备有利于消化吸收,具有降血压和抗氧化等特定生理功能的玉米肽。适度变性的玉米蛋白易被Protamex~蛋白酶水解,热处理的时间和温度均影响玉米蛋白的变性程度,经温度85℃热处理60min的玉米蛋白的水解度最大。相同的水解时间,在pH值为9.0,温度60℃时,该酶对变性玉米蛋白的水解度最大,在此条件下酶解60min,消耗NaOH(5mol/L)16.3mL,玉米的水解度为32.5%,肽的得率为51.2%。SephadexG-25对玉米蛋白水解产物的分离结果表明,Protamex~蛋白酶水解玉米蛋白可生成分子量不同的5个组分。

Preparation of Active Peptides by Hydrolysis of Corn Gluten with Protamex~

Biological active peptides with full nutrition,easy absorption,lowering blood pressure and anti-oxidation were produced with the process of limited hydrolysis of corn gluten by Protamex. Denatured corn gluten was easily hydrolyzed by proteinase. The degree of corn gluten denaturalization was affected by time and temperature of heat treatment,the optimal parameter for corn gluten denaturalization was 85,60 min. The optimal parameters for corn gluten hydrolysis by Protamex was 60 ℃,pH 9.0,60 min,Under this condition,oligo-peptide yield was up to 51.2%. Sephadex G-25 gel filtration chromatography result indicated the hydrolysate of corn gluten consisted five fractionation.