| 鳙鳃凝集素的分离纯化及理化性质的研究 |
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| 引用本文： | 盘赛昆,汤坚,顾小红,杨超. 鳙鳃凝集素的分离纯化及理化性质的研究[J]. 水产科学, 2009, 28(12) |
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| 作者姓名： | 盘赛昆 汤坚 顾小红 杨超 |
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| 作者单位： | 江南大学食品学院,江苏,无锡,214122;淮海工学院,海洋学院,江苏,连云港222005;江南大学,食品科学与技术国家重点实验室,江苏,无锡,214122;淮海工学院,海洋学院,江苏,连云港222005 |
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| 摘 要： | 利用DEAE-Sepharose FF离子交换和Sephacryl S-200 HR及Superdex 200 10/300 GL分子筛层析技术,从鳙鳃组织中分离纯化到一种岩藻糖专一的凝集素,命名为GANL.在还原SDS-PAGE电泳上显示单一蛋白染色带,其亚基相对分子质量为37 kD.经Superdex 200凝胶过滤层析测得其天然相对分子质量为220 kD.GANL的中性糖含量为13.4%.因此,GANL作为一种由相同亚基组成多亚基糖蛋白.GANL对兔红细胞有专一的凝集活性,其凝集活性不依赖Ca~(2+).在被测的单糖、双糖及糖蛋白中,仅岩藻糖能抑制其凝集活性.氨基酸分析表明GANL的Asp、Glu、Leu、Val、Lys的含量较高,Cys-S的含量为0.81%.GANL的最适pH为8～9;具有很高的热稳定性,最适温度为50 ℃.
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| 关 键 词： | 凝集素 鳙 鳃 分离纯化 性质 |
Purification and Characterization of Lectin from Gill of Bighead Carp(Aristichthys nobilis) |
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| PAN Sai-kun,TANG Jian,GU Xiao-hong,YANG Chao. Purification and Characterization of Lectin from Gill of Bighead Carp(Aristichthys nobilis)[J]. Fisheries Science, 2009, 28(12) |
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| Authors: | PAN Sai-kun TANG Jian GU Xiao-hong YANG Chao |
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| Affiliation: | PAN Sai-kun1,2,TANG Jian3,GU Xiao-hong3,YANG Chao2(1.School of Food Science , Technology,Jiangnan University,Wuxi 214122,China,2.School of Marine Science , Technology,Huaihai Institute of Technology,Lianyungang 222005,3.State Key Laboratory of Food Science , Technology,China) |
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| Abstract: | A fucose-binding lectin from gill of bighead carp(Aristichthys nobilis)was purified and characterized.The purification procedure consisted of extracting soluble proteins in 25 mmol/L Tris-HCl buffer(pH8.5),separating on DEAE-Sepharose FF ion exchange columns followed by gel filtration chromatography on Sephacryl S-200 HR and Superdex 200 10/300 GL.The purified lectin designated GANL showed a single protein band with an apparent molecular mass of 37 kD when submitted to SDS-polyacrylamide gel electrophoresis... |
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| Keywords: | lectin bighead carp Aristichthys nobilis gill purification characterization |
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