山东省部分桑树栽培品种桑叶中的1-脱氧野尻霉素含量测定

为了选择适合开发桑叶药用及保健产品的桑树品种,以山东蚕区栽培的具有代表性的59个桑树品种为材料,采用反相高效液相色谱法测定各品种春季桑叶中的1-脱氧野尻霉素(DNJ)含量。结果表明,不同桑树品种间的DNJ含量存在极显著差异,含量最高和含量最低的桑树品种之间相差3.2倍,鲁桑品种沂源黄鲁和白桑品种红玛瑙桑叶中的DNJ含量较高,质量分数分别达到0.409 7%和0.382 8%。综合考虑各品种的桑叶DNJ含量以及单位面积桑园桑叶年产量和抗逆性等因素,认为青黄桑和选792两个桑品种的实用价值比较高,适合直接作为桑叶DNJ开发专用桑品种或育种材料。

Determination of 1-Deoxynojimycin Contents in Leaves of Mulberry Cultivars in Shandong Province

In order to select mulberry cultivars suitable for development of medicinal and healthcare product prepared with mulberry leaves,the contents of 1-deoxynojimycin(DNJ) in leaves of representative 59 mulberry varieties cultivated in sericultural areas of Shandong Province in spring were determined by reversed-phase high performance liquid chromatography(RP-HPLC).The results indicated that the contents of DNJ in different mulberry cultivars had extremely significant difference.The ratio between maximum and minimum DNJ content can reach 3.2.DNJ contents in leaves of Yiyuanhuanglu of Morus multicaulis Perr.and Hongmanao of Morus alba Linn.were relatively higher,with mass fraction as high as 0.409 7% and 0.382 8% respectively.In comprehensive consideration of DNJ content in mulberry leaves,annual mulberry leaf yield per unit area of mulberry field and resistance against environmental stresses,Qinghuangsang and Xuan 792 had higher practical value,being appropriate special mulberry varieties or breeding materials for development of mulberry leaf DNJ.