| 葡萄叶片蛋白质双向电泳技术体系的建立 |
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| 引用本文： | 张玉洁,张军科,罗世杏. 葡萄叶片蛋白质双向电泳技术体系的建立[J]. 北方园艺, 2010, 0(13): 147-151 |
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| 作者姓名： | 张玉洁 张军科 罗世杏 |
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| 作者单位： | 西北农林科技大学,园艺学院,农业部西北园艺植物种质资源利用重点开放实验室,陕西,杨凌,712100;西北农林科技大学,园艺学院,农业部西北园艺植物种质资源利用重点开放实验室,陕西,杨凌,712100;西北农林科技大学,园艺学院,农业部西北园艺植物种质资源利用重点开放实验室,陕西,杨凌,712100 |
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| 基金项目： | 陕西省重大攻关资助项目 |
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| 摘 要： | 现对葡萄叶片中总蛋白质的双向电泳技术体系进行了初步探讨,比较了不同等电聚集程序、不同pH胶条以及不同SDS-PAGE凝胶浓度的双向电泳效果。结果表明:葡萄叶片蛋白质使用等电聚焦程序B、pH范围为4~7的胶条和12%的SDS-PAGE凝胶可以得到较为理想的双向电泳分析结果;采用7 cm胶条最大可以分离出143个有效蛋白质点。
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| 关 键 词： | 葡萄 蛋白质组 双向电泳 |
Study on the Conditions of 2-DE Analysis of Proteome in Grape Leaveas |
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| ZHANG Yu-jie,ZHANG Jun-ke,LUO Shi-xing. Study on the Conditions of 2-DE Analysis of Proteome in Grape Leaveas[J]. Northern Horticulture, 2010, 0(13): 147-151 |
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| Authors: | ZHANG Yu-jie ZHANG Jun-ke LUO Shi-xing |
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| Affiliation: | ZHANG Yu-jie,ZHANG Jun-ke,LUO Shi-xing (College of Horticulture,Northwest Agricultural and Forestry University,Key Lab of Northwestern Horticultural plant Germplasm Resource Application of Ministry of Agriculture,Yangling,Shaanxi 712100) |
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| Abstract: | The method system of 2-dimensional electrophoresis(2-DE)of protein in grape leaves was studied,the results of 2-dimensional electrophoresis from different procedures of isoelectric focusing,different immobilized pH gradients strips and with different SDS-PAGE gel concentration were compared.The results showed that isoelectric focusing procedure B,immobilized pH gradients(IPG)strip with the linear range of pH 4~7 and 12% SDS-PAGE gel were the better choice,more than 143 protein spots were separated in one ru... |
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| Keywords: | grape proteome two-dimensional electrophoresis |
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