尼罗罗非鱼胰岛的显微和亚显微结构

应用光镜、电镜及特殊染色等技术对尼罗罗非鱼(Tilapia nilotica)的胰岛及其细胞类型和形态进行了详细的研究。尼罗罗非鱼的胰脏与肝脏混合存在，属于肝胰脏。胰岛散布在胰腺内，无固定的形态，胰岛细胞的数量与类型因胰岛的大小而异。用Gomori醛复红特殊染色法，见尼罗罗非鱼胰岛有三种细胞：A细胞胞质中有黄色颗粒；B细胞胞质中有紫红色的颗粒；D细胞胞质内有浅绿色或淡灰色的颗粒。电镜下，B细胞的分泌颗粒较大,芯的形态多样，电子密度差异大,芯与界膜之间常有较大的间隙；A细胞分泌颗粒形态不规则，芯的大小不等，界膜与芯之间无间隙；D细胞分泌颗粒较多，芯的电子密度较低，界膜与芯之间无间隙。此外，胞质内可见较大的线粒体。

The microscopic and submicroscopic structure on the pancreas islet in Tilapia nilotica

The type and morphology of the pancreas islet as well as its cells in Tilapia nilotica were studied in detail by using the microscope, electron microscope and special staining techniques. The pancreas in Tilapia nilotica which combine with liver, belongs to hepatopancreas. The pancreas islet, without fixed form, scatter in the pancreas. The number and type of pancreatic endocrine cells change following the size of pancreatic islet. With the help of Gomori aldehyde fuchsine stain, we can find three kinds cells in pancreas islet. There are yellow granules in A cell, purplish red granules in B cell and green or light gray granules in D cell.Under the electron microscope,secretic granules in B cell are fairly big, cores are various. There is fairly big gap between the core and the limiting membrane; the center of secretic granules in A cell exist compact core and there is no gap between the core and the limiting membrane; Secretic granules in D cell are too much, electron density of granules is lower;And there is no gap between the core and the limiting membrane;What's more, fairly big chondriosome can be seen in the cell.