花椰菜幼苗叶片抗氧化酶系统与抗黑腐病关系的研究

 在盆栽条件下,花椰菜抗病品种雪峰和感病品种2003X-106的幼苗在接种黑腐病菌后,叶片组织内O₂^·产生速率,SOD、POD、PAL活性,MDA含量和膜透性都有不同程度的提高和增加。与2003X-106相比,雪峰的O₂^·产生速率和SOD、PAL活性的增加更为显著,POD活性也要高于2003X-106;而MDA含量和电导率的增幅要明显低于2003X-106。表明抗病品种雪峰比感病品种2003X-106具有更强的抗黑腐病能力和抗氧化能力。

Changes of anti-oxidative system in cauliflower seedlings infected by black rot and their relationships with the resistance to black rot

The dynamic changes including O₂^· generation rate, superoxide dismutase (SOD), peroxidase (POD) and phenylalanine ammonialyase (PAL) activities, malondialdehyde (MDA) content and membrane leakage (relative conductance rate) in cauliflower seedling leaves, which were infected by Xanthomonas campestris pv. campestris were studied. It was found that all of O₂^· generation rate, SOD, POD and PAL activities, MDA content and relative conductance rate increased more or less after inoculation in both the resistant variety (Xuefeng) and the susceptible one (2003X-106). However, Xuefeng showed a higher level of O₂^· generation rate, SOD, POD and PAL activities and lower changing rate of MDA content and relative conductance rate than 2003X-106. These results demonstrate that the resistant variety (Xuefeng) is provided with stronger defensibility to black rot and greater ability against membrane lipid oxidation.