玉米自交系愈伤组织诱导及植株再生研究

以玉米自交系7922、H99、P2、Mo17、吉846、吉63等的幼胚为外植体诱导愈伤组织，研究了不同培养基、不同激素种类和浓度及不同碳源的种类和浓度对愈伤组织诱导的影响。结果表明：NB培养基的胚性愈伤组织诱导率最高；对于不同基因型玉米自交系2,4-D浓度为2 mg/L时胚性愈伤组织诱导率均最高；在以20 g/L蔗糖和20 g/L葡萄糖及30 g/L蔗糖和10 g/L葡萄糖作碳源的诱导培养基上愈伤组织诱导率最高，但不能长期继代使用。对不同激素组合对玉米愈伤组织再生分化的影响进行了探讨。

Study on Induction Embryogenic Callus and Plant Regeneration in Maize Inbred Lines

In experiment, factors influencing the callus induction of immature embryos in maize inbred lines 7922, H99, P2, Mo17, Ji846, Ji63, etc. Such as different media, different concentrations of phytohormone, different carbon sources and their concentrations were studied. The results showed that NB was optimal media of callus induction on immature embryos in different maize inbred lines, concentration of 2,4-D 2 mg/L could obviously enhance inducing frequency, concentration of sucrose 20 g/L and 20 g/L glucose or sucrose 30 g/L and 10 g/L glucose was the better conditions of callus induction, but could not be used in long period. More over, this paper discussed the effects of different hormone on regeneration of maize callus.