| 菜豆几丁质酶基因转化小麦的研究 |
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| 引用本文： | 刘伟华,李文雄,刘绵红,李新玲,张燕妮,赵欣.菜豆几丁质酶基因转化小麦的研究[J].东北农业大学学报,2001,32(2):111-116. |
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| 作者姓名： | 刘伟华 李文雄 刘绵红 李新玲 张燕妮 赵欣 |
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| 作者单位： | 1. 东北农业大学农学院
2. 哈尔滨师范大学生物系 |
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| 摘 要： | 利用农杆菌二元载体转化法和花粉管通道法 ,以菜豆几丁质酶基因转化小麦东农 774 2。农杆菌感染后的愈伤组织以 G4 183 0 mg· L-1筛选 5周 ,经 PCR和 PCR- Southern杂交检测 ,有0 .3 8%的愈伤获得转化。花粉管通道法转化小麦共操作小花 3 85朵 ,其中有 68.3 0 %的小花结实 ,经 PCR和 PCR- Southern杂交检测 ,获得转基因植株的转化频率为 0 .52 %。
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| 关 键 词： | 几丁质酶 基因 转化 小麦 |
| 文章编号： | 1005-9369(2001)02-0111-06 |
| 修稿时间： | 2000年9月19日 |
Study of transforming bean chitioase gene into the wheat |
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| LIU Wei hua,LI Wen xiong,LIU Jin hong LI Xin ling,ZHANG Yan ni,ZHAO Xin.Study of transforming bean chitioase gene into the wheat[J].Journal of Northeast Agricultural University,2001,32(2):111-116. |
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| Authors: | LIU Wei hua LI Wen xiong LIU Jin hong LI Xin ling ZHANG Yan ni ZHAO Xin |
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| Institution: | LIU Wei hua,2LI Wen xiong,1LIU Jin hong 1LI Xin ling,2ZHANG Yan ni,2ZHAO Xin |
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| Abstract: | Bean chitionase gene was transformed into spring wheat Culfivar Dongnong 7742 by Agrobacterium tumefacicns-mediated transformation and pollen tube pathway.The callia infected with Agrobacterium tumefaciens has been selected for five weeks by G418 30mg/L,and tested by PCRand PCR-southern Blotting.The results indicated that 0.38% callia has been transformed successfully.However,385 little buds have been operated using pollen tube pathway,then 68.30% buds breeded.The frepuency of transgenic plants was 0.52% by PCR and PCR-suthern Blotting testing. |
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| Keywords: | Chitinase gene Transformation Wheat |
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