广州一号桉树高效组培再生体系的建立

以广州一号桉树优良无性系无菌苗茎段为外植体,通过对噻唑基脲类新型分裂素(N-phenyl-N’-6-(2-chlorobenzothiazol)-yl]urea,PBU)等多种不同质量浓度植物生长调节剂组合的优化,进行愈伤组织诱导及再生研究。结果表明,在添加了2 mg.L-1PBU和0.06 mg.L-1IAA的改良MS培养基上,茎段外植体5 d后愈伤组织诱导率达100%;将愈伤组织接种在添加1 mg.L-16-BA和0.1 mg.L-1NAA的MS培养基上,诱芽率高达90%;随后,在添加了0.2 mg.L-1PBU与0.05 mg.L-1NAA的1/2MS培养基上诱导芽伸长,用1/2MS培养基附加0.2 mg.L-1IBA诱导生根,炼苗15 d后,以质量m(黄心土)∶m(腐殖质土)=1∶1为基质进行移栽,得到完整再生植株,成活率高达95%。

Establishment of Tissue Culture Regeneration System of Eucalyptus Guangzhou NO.1

The callus induction and shoot regeneration of elite clone of Eucalyptus Guangzhou No.1 were studied systematically using a variety of combinations of different plant growth regulators,such as N-phenyl-N′-6-(2-chlorobenzothiazol)-yl] urea(PBU).Sterile clones of seedling stem segments were used as explants and cultured in a modified MS medium supplemented with the synthetic growth regulator 2mg·L-1 PBU and 0.06mg·L-1 IAA.After cultivation for 5 days,100% of explants formed callus.Calluses were transferred to MS medium containing 1mg·L-1 6-BA and 0.1mg·L-1 NAA to induce bud formation,and 90% of the callus induced by PBU exhibited adventitious bud formation.Shoot elongation was then stimulated on 1/2MS supplemented with 0.2mg·L-1 PBU and 0.05mg·L-1 NAA for 20 days.For rooting,the elongated shoots were cultivated on root induction medium containing 0.2mg·L-1 IBA.After 15 days of acclimatization,the survi-val rate of seedlings reached 95% with a mixture of yellow soil and humus soil(1:1 by volume) as a substrate for transplanting,and fully mature plants were obtained.