| 甘蓝品种的AFLP指纹鉴别图谱分析 |
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| 引用本文： | 宋顺华,郑晓鹰.甘蓝品种的AFLP指纹鉴别图谱分析[J].分子植物育种,2006,4(Z1):51-54. |
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| 作者姓名： | 宋顺华 郑晓鹰 |
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| 作者单位： | 北京市农林科学院蔬菜研究中心，北京市农林科学院蔬菜研究中心 北京，100089，北京，100089 |
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| 摘 要： | 本试验采用AFLP技术分析了来自全国9个栽培地区的44个甘蓝主栽品种,共筛选了40对E 3/M 3引物组合,多态性条带的数量从0条到15条不等。其中引物组合E-AAC/M-CTA是甘蓝品种中多态性最高的引物,有15条多态性条带,多态性条带的百分率为30%,但该引物不足以区分44个供试的甘蓝品种。同时筛选了11组E 2/M 3对引物组合,其中引物组合E-AG/M-CTC产生了13条清晰的多态性条带。以E-AAC/M-CTA和E-AG/M-CTC这两对引物组合的多态性条带构建了44份甘蓝品种材料的指纹图谱,此指纹图谱可以将供试的44份材料一一区分。
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| 关 键 词： | AFLP分子标记 结球甘蓝 品种 |
Analysis of AFLP Fingerprinting in Cabbage Cultivars Testing |
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| Song Shunhua Zheng Xiaoying Beijing Vegetable Research Center,Beijing Academy of Agricultural and Forestry Science,Beijing.Analysis of AFLP Fingerprinting in Cabbage Cultivars Testing[J].Molecular Plant Breeding,2006,4(Z1):51-54. |
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| Authors: | Song Shunhua Zheng Xiaoying Beijing Vegetable Research Center Beijing Academy of Agricultural and Forestry Science Beijing |
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| Institution: | Song Shunhua Zheng Xiaoying* Beijing Vegetable Research Center,Beijing Academy of Agricultural and Forestry Science,Beijing,100089 |
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| Abstract: | In this study, AFLP was used to evaluate the genetic polymorphism of cabbage cultivars, including 44 cultivars collected from 9 different culture regions. 40 pairs of E 3/M 3 AFLP primers were screened. The range of polymorphic bands detected by individual pair of primer were from 0 to 15. E-AAC/M-CTA which detected 50 fragments has particularly high efficiency detecting 15 polymorphic fragments (30%), but it was not able to distinguish all of the 44 accessions. Then 11 pairs of E 2/M 3 AFLP primers were screened. E-AG/M-CTC detected 13 polymorphic fragments. 44 cabbage cultivars testing fingerprinting has been established with these polymorphic bands combined E-AAC/M-CTA and E-AG/M-CTC, which was able to distinguish all of the 44 accessions. |
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| Keywords: | AFLP molecular markers Cabbage (Brassica oleracea L ) Cultivars |
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