水稻白叶枯病菌毒素对水稻不育系珍汕97A的专化毒性

 采用乙酸乙酯法提取对珍汕97不育胞质具有专化毒性的白叶枯病菌AH28、GX50和非专化毒性的OS14菌株的毒素。以不同浓度的毒素处理珍汕97不育系和保持系的种子或幼苗,可引起水稻叶片褪绿和细胞坏死,产生与接种病菌相似的症状,在蚕豆叶片上形成过敏性坏死斑;毒素可导致水稻幼苗萎蔫、胚根胚芽生长受抑和根冠细胞死亡;毒素的毒性与毒素浓度和产毒素菌株的致病力成正比;专化性毒素AT对珍汕97不育系的毒害程度显著高于对保持系的毒害。

ON THE SPECIFIC TOXICITY OF TOXIN PRODUCED BY XANTHOMONAS ORYZAE PV. ORYZAE TO CYTOPLASMIC MALE STERILE RICE ZHENSHAN 97A

The toxins produced by strains of Xanthomonas oryzae pv. oryzae Ah28,GX50 and OS14 were extracted with ethylacetate.Seeds and seedlings of Zhenshan97 cms line(A) and its maintainer (B) were treated with toxins.The toxin caused discoloration and cell death of rice leaves which were similar to those of the pathogen.The toxin also induced necrotic hypersensitive reaction of broad bean leaves and wilting of rice seedlings.In addition,the toxin could significantly inhibit the growth of roots and shoots and kill the root cap cells of rice.The toxicity of toxin was possitively correlatede with the concentration and virulence of pathogen.The toxins produced by strains which were specific to Zhenshan 97 A also showed stronger toxicity to male sterile cytoplasm than to normal cytoplasm.