用RAPD研究我国杨生褐盘二孢菌的群体分化

 杨生褐盘二孢菌(Marssonina brunnea)是引起我国杨树黑斑病的最主要病原。病原菌的形态学和致病性分析已经证明这个种有分化现象。本研究从我国不同地区不同寄主上收集了42个杨生褐盘二孢菌菌株,用随机扩增多态性DNA (RAPD)研究了它们之间的遗传多样性。聚类分析根据80%的相似性把42个菌株分成3个组(A、B和AB组)。A组中包含有4个分离自毛白杨的菌株;B组中含37个自青杨派和黑杨派分离的菌株;AB组中仅含有1个分离自响叶杨的菌株。推断AB组菌株可能是A组菌株向B组菌株进化的一个过渡类型。

DIFFERENTIATION OF MARSSONINA BRUNNEA IN CHINA BY RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) ANALYSIS

Marssonina brunnea is the causal agent of marssonina leaf spot of poplar and occurs in most regions of China. Differences in morphology of conidial germination and pathogenicity among isolates from various hosts suggested the existence of strains or specialized forms of this fungus. Random amplified polymorphic DNA markers (RAPDs) were used to assess genetic diversity among 42 isolates of M. brunnea collected from different poplars in Jiangsu,Henan,Shandong, Shaanxi, Beijing and Jilin provinces of China. Relationships among isolates were determined using cluster analyses. Four isolates from Populus tomentosa were placed in one group (group A) and Thirty-seven isolates from the poplars of section aigeiros and section tacamahaca in another group (group B). The isolate from P. adenopoda was placed in the third group (group AB). The genetic relationship of these group was discussed.