盾壳霉所产抗细菌物质的特性

 在研究盾壳霉(Coniothyrium minitans)生态特性中,我们首次发现了该菌可产生抗细菌物质。本试验测定了盾壳霉产生的抗细菌物质对13种(或类型)细菌的抑制作用。并对该物质的热稳定性、酸碱稳定性、纸层析和纸电泳迁移作了研究。结果表明:该物质可抑制多种革兰氏阴性和阳性细菌,尤其对水稻白叶枯病菌(Xanthomonas oryzae pv.oryzae)的抑制作用最好;可耐121℃ 1h高温处理;在酸性条件下稳定,在pH值大于或等于11的碱性溶液中丧失活性;纸层析和纸电泳分析表明该物质含有2种有效成份,皆为非水溶性物质,其中一种是中性化合物,另一种是弱酸性化合物。

STUDIES ON THE PROPERTIES OF ANTIBACTERIAL SUBSTANCE PRODUCED BY CONIOTHYRIUM MINITANS

We discovered that Coniothyrium minitans has the ability to produce antibacterial substance when we studied its ecological properties.In this paper,13 bacteria species (or types) were used to test their senstivities to the antibacterial substance produced by Coniothyrium minitans. The heat-stability and acid-stability of this substance were also examined. To analysis this substance,the movement of this substance on the paper was observed with the use of paper chromatography, and paper electrophoresis. The results showed that the antibacterial substance inhibited the growth of both Gram positive and Gram negative bacteria, especially showed strong activity against Xanthomonas oryzae pv. oryzae, one of the most important rice pathogens. It could tolerate heat (1 h under 121℃) and acid treatment. According to paper chromatographic analysis and paper clectrophoresis analysis,there were two valid components in this antibacterial substance,the one was neutral,and the other was of weak aidity.