| 不同激素浓度对黄芪茎段组织培养的影响 |
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| 引用本文： | 霍晓兰,闫敏,孟全业,张强.不同激素浓度对黄芪茎段组织培养的影响[J].山西农业科学,2009,37(3). |
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| 作者姓名： | 霍晓兰 闫敏 孟全业 张强 |
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| 作者单位： | 1. 山西省农业科学院土壤肥料研究所,山西,太原,030031
2. 山西省农业种子总站,山西,太原,030001 |
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| 基金项目： | 西省农业科学院青年基金 |
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| 摘 要： | 以蒙古黄芪茎段为外植体,MS为基本培养基,设计不同浓度的IAA,NAA和6-BA等单因子和不同比例的细胞分裂素组合试验。结果表明,在芽的诱导增殖过程中,MS+NAA0 mg/L+6-BA1.0 mg/L的培养基效果最好;在诱导植株茎叶壮实高大增殖过程中,MS+NAA 0 mg/L+6-BA 0.3 mg/L的培养基效果较好,诱导率分别为89.2%和82.8%;生根培养过程中,MS+IAA2.0 mg/L的培养基效果较好,诱导生根率为55.9%。
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| 关 键 词： | 黄芪 茎段 组织培养 |
Effects of Different Plant Hormone Concentrations to Tissue Culture Stem of Astragalus Membranaceus Bge |
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| HUO Xiao-lan,YAN Min,MENG Quan-ye,ZHANG Qiang.Effects of Different Plant Hormone Concentrations to Tissue Culture Stem of Astragalus Membranaceus Bge[J].Journal of Shanxi Agricultural Sciences,2009,37(3). |
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| Authors: | HUO Xiao-lan YAN Min MENG Quan-ye ZHANG Qiang |
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| Abstract: | Stalk segments of Astragalus membranaceus Bge were cultured as explants on medium supplemented with plant hormone in different concentrations.The results showed that Murashige and Skoog(MS) medium supplemented with NAA 0 mg/L,6-BA 1.0 mg/L was the most effective medium for the differentiation and multiplication of the shoots.In the process of proliferation for plant tall and sturdy stems and leaves,MS + NAA 0 mg /L +6-BA 0.3 mg /L of the medium better.The induction rate was 89.2% and 82.8%.The optimum rooting medium was MS+IAA 2.0 mg/L.The induction rate of root was 55.9%. |
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| Keywords: | Astragalus membranaceus Bge Stem Tissue culture |
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