| 黄牛睾丸特异乳酸脱氢酶-C基因选择性剪接体的克隆与序列分析 |
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| 引用本文： | 洪键,郑玉才,贺庆华. 黄牛睾丸特异乳酸脱氢酶-C基因选择性剪接体的克隆与序列分析[J]. 华北农学报, 2009, 24(3) |
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| 作者姓名： | 洪键 郑玉才 贺庆华 |
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| 作者单位： | 西南民族大学生命科学与技术学院,四川,成都,610041;盐城师范学院生命科学与技术学院,江苏盐城,224002;西南民族大学生命科学与技术学院,四川,成都,610041 |
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| 基金项目： | 四川省青年科技基金资 |
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| 摘 要： | 采用RT-PCR方法从黄牛睾丸组织中克隆睾丸特异乳酸脱氢酶.C(LDH-C)基因的cDNA序列,结果发现2种Ldhc剪接体,根据Ldhc基因在进化上的保守性,参照普通牛Ldhc基因结构分析了黄牛Ldhc基因的选择性剪接体的结构,结果显示,剪接体存在外显子缺失:其中剪接体1缺失第7个外显子,剪接体2缺失第4和第7两个外显子.因为LDH-CA酶的NAD+结合结构域由Ldhc的外显子2-4编码,酶的活性中心由外显子4编码.所以剪接体1包含完整酶的NAD+结合结构域和酶的活性中心,剪接体2失去了大部分NAD+结合结构域和酶的活性中心.对黄牛睾丸组织和精子LDH同工酶的电泳分析未检测到潜在的Ldhc剪接体的表达,推测睾丸组织Ldhc选择性剪接的功能是调控该基因在睾丸中表达的方式之一.
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| 关 键 词： | 黄牛 乳酸脱氢酶-C 睾丸 |
Cloning and Sequence Analysis of Multiple Splice Variants of Lactate Dehydrogenase C in Cattle Testes |
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| HONG Jian,ZHENG Yu-cai,HE Qing-hua. Cloning and Sequence Analysis of Multiple Splice Variants of Lactate Dehydrogenase C in Cattle Testes[J]. Acta Agriculturae Boreali-Sinica, 2009, 24(3) |
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| Authors: | HONG Jian ZHENG Yu-cai HE Qing-hua |
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| Affiliation: | 1.College of Life Science and Technology;Southwest University for Nationalities;Chengdu 610041;China;2.College of Life Science and Technology;Yancheng Teachers University;Yancheng 224002;China |
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| Abstract: | Total RNA was extracted from cattle testes and reverse transcribed and amplified by RT-PCR.The two splice variants of LDH-C were obtained.Compared with the full length ORF of bovine LDH-C,as a result,The two cloned splice variants were missing exons,In detail,variants 1(missing the coding sequence of exon 7);Variant 2(missing the coding sequence of exons 4 and 7)lacks a large part of the NAD binding domain and the entire active site.Native polyacrylamide gel electrophoresis was performed to determine if the... |
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| Keywords: | Cattle Lactate dehydrogenase C Testes |
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