High-level production of recombinant chicken cystatin by Pichia pastoris and its application in mackerel surimi |
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| Authors: | Chen G H Tang S J Chen C S Jiang S T |
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| Institution: | Deaprtment of Food Science and Institute of Marine Biotechnology, National Taiwan Ocean University, Keelung, Taiwan. |
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| Abstract: | A high level of the secreted form of recombinant chicken cystatin was expressed in Pichia pastoris X-33 by chromosomal integration of multiple copies of an expression cassette containing chicken cystatin under the control of glyceraldehyde-3-phosphate dehydrogenase promoter. The inhibition ability of the recombinant for papain-like proteinase was found to correspond to those of natural chicken cystatin. The recombinant cystatin substantially inhibited the proteolysis of myosin and gel softening, which consequently improved the gel properties of mackerel surimi. |
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