N605ab,a specific molecular marker for <Emphasis Type="Italic">Phytophthora infestans</Emphasis>, distinguishes three genotypes in Japan |
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| Authors: | Seishi Akino Yuko Shirasawa Norio Kondo Shigeo Naito |
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| Institution: | (1) Laboratory of Plant Pathology, Graduate School of Agriculture, Hokkaido University, Sapporo 060-8589, Japan;(2) Present address: Food and Agricultural Materials Inspection Center, Odori Nishi 10-4-1, Sapporo 060-0042, Japan |
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| Abstract: | Random amplified polymorphic DNA (RAPD) analysis using the OPG-06 primer generated specific patterns for Japanese genotypes
US-1, JP-1, and a new A1 (JP-2, JP-3, and JP-4) of Phytophthora infestans. N605, a specific RAPD fragment, was cloned and sequenced. PCR primers BD1/BD2 were constructed based on the N605 sequence
and were used to clarify the genotypes. PCR products using the BD1/BD2 primers (N605ab marker) easily distinguished the new
A1 from US-1 and JP-1. This technique provides a simple and effective method for rapid genotype discrimination that can be
used in ecological experiments and forecasts for the occurrence of late blight. |
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| Keywords: | RAPD Population genetics Potato late blight |
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