无病毒百合组培种球快速繁殖体系的建立立

本研究以铁炮百合(Lilium Longiflorum)感病种球为材料，对热处理后的种球进行了培养基附加物和移栽基质的优化，建立了无病毒组培种球快速繁殖体系。结果表明：以MS为基本培养基，当附加植物激素NAA和KT时能促进子球再生，再生率达86%以上， 在0.2mg?L-1NAA和 0.4mg?L-1 KT的培养基中平均再生子球数显著增多；附加5mg?L-1多效唑培养基能促进子球增大，3mg?L-1多效唑有利于子球再分化；活性炭对子球增大无明显影响，但适当浓度的活性炭能提高培养子球的品质；高浓度蔗糖能促进子球增大，但对子球再分化有抑制影响。蛭石:土、珍珠岩以及珍珠岩：土作为基质时，均能达到较高的成活率和株高，但在珍珠岩：土为3：1作为基质移栽组培球，其成活率和生长状况最佳。37℃热处理随着处理天数的增加，处理后培养鳞片的成活率和子球再生率显著下降，但处理20天子球再生率只能达40%左右，但LSV检出率明显低于未处理的种球，脱毒率达95%以上。

Establishment of In Vitro Rapid Propagation System on Virus-free Bulblets of Lilium spp.

Virus-free in vitro bulblet rapid propagation system was established using Lilium Longiflorum as experimental material. The results showed that the best medium for bulblets regeneration from bulb scales was MS＋KT 0.4 mg/L＋NAA0.2 mg/L. Optimum bulblet enlargement was observed in medium with Paclobutrazol 5mg/L, while Paclobutrazol in concentration 3 mg/L was propitious to bulblet formation. The quality of bulblets can be enhanced in the medium with appropriate concentration of AC. Medium with 90g/L sucrose can promote bulblet enlargement but inhibit bulblet formation. The best matrix for the highest surviving rate and best growth situation was pearlite/soil（3 ： 1）. About 50% bulb scales survived and regenerated after the treatment of high temperature 37℃ for 20 days, the effectiveness of eliminating virus with the method got to more than 95%.