秋水仙素对当归多倍体愈伤组织诱导的影响因素分析

在添加浓度为１００ｍｇ／Ｌ秋水仙素的继代培养基上，对体积约为０．５ｃｍ３和０．３ｃｍ３的当归愈伤组织分别处理１０ｄ、２０ｄ、２６ｄ和３５ｄ，并在处理结束后的第１ｄ和第１０ｄ取样，观察细胞倍性的变化趋势。结果表明：处理时间对４ｘ细胞比例有极显著的影响；在处理结束后的恢复生长中，二倍体细胞明显增加，八倍体细胞显著减少，四倍体细胞则保持稳定；在长时间的诱导处理中，接种物团块大小对加倍效果无显著影响

Factors Affecting on Chromosome Doubling of Callus of Angelica Sinensis by Colchicine Treatment

The changes of different ploidy cells in callus of Angelica sinensis sized about 0.5 cm 3 or 0.3 cm 3 and then treated by Colchicine with concentration of 100mg/L being added in the MS subculture medium for 10, 20, 26 and 35 days, respectively, were measured through examining the chromosome numbers in samples taken at 0 and 10 days after treatment. It was showed that durations of the treatment had significant effects on the percentages of 4x cells (31 26%～44 76%, P <0.01) and slight effects on 2x cells (23.91%～29.95%, P >0.05) and 8x cells (38.47%～44.38%, P >0.05) in the samples at 0 day. Therefore the changes of the relative percentages of these three kinds of cells in the callus subcultured for 10 days after the treatment were the 2x cells significantly increased ( P >0.01), 8x cells decreased ( P >0.01) and 4x cells remained at the similar levels compared to the observations at 0 day. The size of callus did not affect the chromosome doubling in the long term treatment by Colchicine.