提高白菜离体培养植株再生频率的研究

选用 7个白菜类蔬菜栽培品种和 1个杂交亲本进行离体培养植株再生试验 ,筛选出最佳培养基配方为 1/2倍NH 4 浓度的MS培养基附加BAP 2mg/L、NAA 0 .4 5mg/L和AgNO37.5mg/L ,大幅度提高了植株的再生频率 ,高达 84 .8% ,大大缩短成苗周期 ;子叶—子叶柄接种后 ,最早的仅需 6d即可分化出不定芽 ,从接种到再生苗的移栽最快的仅需 4 0d ,平均一批约需 50d ,达到了高频快速的要求 ;该培养基对白菜类蔬菜的不同品种有着较广的适应性。对不定芽的发生过程进行组织学观察表明 ,在本试验条件下 ,白菜离体培养植株再生方式为外植体直接出芽。再生苗生根后移栽的成活率可达 95%以上。

Enhancement of Plant Regeneration Frequency of in Vitro Cultured Chinese Cabbage

An efficient and fast method for plant regeneration from petiole explants with cotyledon was developed in Chinese cabbage( Brassica campestris ssp. chinensis Makino).A MS medium with half strength of NH 4 concentration and supplemented with BAP 2mg/L,NAA 0.45mg/L and AgNO 3 7.5mg/L was found to be optimum for Chinese cabbage in obtaining the high frequency of shoot regeneration.The frequency of shoot regeneration of 7 Chinese cabbage cultivators and 1 parentage reached as high as 84.8%.The little shoots could be observed 6 days after inoculation and shoots were formed directly.The survival rate of planets after transplanting to soil reached as high as 95%.