水稻淀粉型胚乳细胞发育中核的衰退和水解酶的细胞化学定位

利用透射电子显微镜观察了水稻淀粉型胚乳细胞发育中核的衰退和水解酶的细胞化学定位。结果表明，随着淀粉型胚乳细胞的发育，细胞核表现出植物PCD的衰退特征：核变形，染色质凝缩，核周腔膨大。胞质中液泡和内质网包裹凝缩的染色质。淀粉型胚乳细胞发育的中、后期，水解酶（酸性磷酸酶、G-6-P酶、ATP酶、Ca²⁺-ATP酶）在核上表现较高的活性。上述结果表明，淀粉型胚乳发育经历PCD过程。

Degeneration of Nucleus and Ultracytochemical Localization of Hydrolytic Enzymes during Starchy Endosperm Development in Rice

The degeneration of nucleus and ultracytochemical localization of hydrolytic enzymes during starchy endosperm development in rice were observed using transmission electron microscopy.With the developing of starchy endosperm cells,several distinct morphological features of PCD existed in nucleus,such as deformation of nucleus,condensation of chromatin,swelling of perinuclear space.Electron dense granules similar to the condensed chromatin were encroached by the vacuole and the dilated endoplasmic reticulum fragments.Localization of hydrolytic enzymes,such as acid phosphatase(AcPase),ATPase,glucose-6-phosphatase(G-6-Pase),Ca~(2 )-ATPase,demonstrated high enzyme activity in the nucleus at the middle and late stages of starchy endosperm development.These results suggest that rice starchy endosperm underwent programmed cell death during its development.