Chromosome number doubling of 2x potato lines with diverse genetic background through tissue culture |
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Authors: | A. Sonnino M. Iwanaga A. henostroza |
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Affiliation: | (1) The International Potato Center (CIP), 5969 Lima, Peru;(2) Present address: C.R.E. Cassaccia, Dept. of Agriotechnologies, ENEA, P.O. Box 2400, Ad 00100 Rome, Italy |
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Abstract: | Summary Twelve 2x potato clones of diverse genetic origin were tested for doubling of their chromosome number by placing stem and petiole segments in the regeneration medium (Hermsen et al., 1981). Eleven of the 12 clones yielded doubled plants (2n=4x=48). The percentages of regenerated tetraploid plants varied from 0 to 77% among the genotypes tested. The high rates of successful doubling from many genetically different clones, demonstrates the applicability of this method as a means to convert valuable 2x potato germplasm into the 4x level. |
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Keywords: | haploid adventitious shoot 2n gametes chimera |
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