Detection of bovine virus diarrhea virus in a live bovine herpes virus 1 marker vaccine

In February 1999, 12 Dutch herds were vaccinated with a live bovine herpesvirus 1 vaccine from which bovine virus diarrhea virus (BVDV) could be isolated. All vaccine batches that were on the Dutch market and that had not yet reached the expiry date were tested for BVDV. In total, seven of 82 batches tested were found positive. Batch numbers TX3607, VB3914, VB3915, VB4046, TW3391, and TV3294 were positive for BVDV type 1, and batch number WG4622 was positive for BVDV type 2. This latter batch induced clinical signs of BVDV in an animal experiment with susceptible animals.