| 青杨派树种雌蕊柱头可授性及最佳授粉时期 |
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| 引用本文： | 王君,康向阳,刘立成,姜鹏,付贵生.青杨派树种雌蕊柱头可授性及最佳授粉时期[J].北京林业大学学报,2009,31(4):30-35. |
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| 作者姓名： | 王君 康向阳 刘立成 姜鹏 付贵生 |
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| 作者单位： | 1 北京林业大学林木育种国家工程实验室，林木花卉遗传育种教育部重点实验室 2 内蒙 古通辽市林业科学研究所 |
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| 摘 要： | 为提高青杨派树种有性杂交和多倍体诱导效率,以小青杨、小叶杨和哲引3号杨等青杨派树种及杂种为材料,用过氧化物酶试纸溶液测试结合授粉观察的方法对其雌蕊柱头可授性及最佳授粉时期进行了研究。结果表明: 在温室水培条件下,青杨派树种花序可授期持续4～5 d; 花序不同部位雌蕊柱头获得可授性的时间不同,基部雌蕊柱头最早获得可授性,而顶部雌蕊柱头获得可授性最晚; 过氧化物酶试纸溶液在具有可授性的柱头表面呈现蓝色阳性反应,在育种实践中,可以应用过氧化物酶试纸溶液快速、有效地对青杨派树种雌蕊柱头可授性进行检测; 3种青杨派树种雌花序柱头最佳授粉期为花序顶部露出芽鳞84～96 h,以此为参照点,对于适时施加理化处理诱导胚囊染色体加倍选育青杨三倍体等工作具有重要的指导价值。
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| 关 键 词： | 青杨派 柱头可授性 过氧化物酶试纸 最佳授粉时期 |
| 收稿时间： | 1900-01-01 |
Stigma receptivity and the optimal pollination period of Populus spp. (Section Tacamahaca) |
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| WANG Jun,KANG Xiang-yang,LIU Li-cheng,JIANG Peng,FU Gui-sheng.Stigma receptivity and the optimal pollination period of Populus spp. (Section Tacamahaca)[J].Journal of Beijing Forestry University,2009,31(4):30-35. |
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| Authors: | WANG Jun KANG Xiang-yang LIU Li-cheng JIANG Peng FU Gui-sheng |
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| Institution: | 1 National Engineering Laboratory for Tree Breeding, Key Laboratory for Genetics and Breeding of Forest Trees and Ornamental Plants of Ministry of Education, Beijing Forestry University, 100083, P. R. China; 2 Forestry Research Institute of Tongliao City, Inner Mongolia , 028000, P. R. China. |
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| Abstract: | The stigma receptivity and optimal pollination period were studied by testing with Peroxtesmo Ko solution and pollen germination on stigmas in Populus pseudo simonii, P. simonii and P. pseudo simonii × P. nigra var. lica, to increase the efficiency of hybridization and polyploid induction in Section Tacamahaca. The results showed that: 1) the stigma receptivity of Section Tacamahaca lasted 4-5 days under a greenhouse condition; 2) the pistils in differen |
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| Keywords: | Section Tacamahaca stigma receptivity Peroxtesmo Ko peroxidase test paper optimal pollination period |
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