补血草种间杂种的形态学观察与SRAP分析

为了更好地开发利用补血草属植物,获得新的种间杂种,并为杂种的进一步筛选以及优异性状新种质的获得打下基础,以二色补血草为母本,黄花补血草为父本进行杂交实验,通过形态学观察及SRAP分子标记对杂交后代进行鉴定和分析.本实验共获得11株杂交后代,经形态学观察表明,杂交种的生长习性与母本一致,花萼色与父本一致,而其叶幅、叶数、茎木质化程度和开花次数等多数性状介于父母本之间;SRAP分子标记对杂交后代及父母本扩增共得到91个DNA片段,其中11个是亲本和杂种后代共有的,80条为多态性带,占88%;并且所有杂种后代均具有亲本的扩增片段,为真杂种.杂种F1-6、7和11号性状优良,可通过组培繁殖形成无性系,作为补血草新类型推广.

Examination of morphological features and SRAP analysis ofinterspecific hybrids in Limonium Mill.

For better use of the field species in Limonium Mill. and sustainable acquisition of new interspecific hybrids for further screening and new germ plasm,the crossing experiment between Limonium bicolor and Limonium aureum were carried out and the offsprings were identified and analysed by morphological features and SRAP molecular markers.In total,11 interspecific hybrids were eventually obtained.The following morphology observation showed that the growth habit of the bybrids was similar to Limonium bicolor,and the calyx color of the hybrids was similar to Limonium aureum.The growth characters of hybrids such as leaf diameter,leaf number,degree of lignification of the stem,state of the branch,flowering time and flowering frequency displayed a number of features intermediate between both parents.In the SRAP experiment,91 DNA bands were obtained,in which 11 bands were presented in both parents and hybrids and 80 polymorphic with a proportion of 88%.All the hybrids displayed the typical bands of parents and their intergenetic status was then implied.The character of F1-6,7 and 11 were excellent.They can propagate by tissue culture and popularize as a new omamental.