Effect of propolis water extract on apoptosis of human umbilical vein endothelial cells in vitro |
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Authors: | ZHANG Yun-xiang WANG Jia-fu LI Wei CHEN Bin |
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Affiliation: | 1.Department of Pathology,Taishan Medical University, Taian 271000, China;2.Department of Pathology, Weifang People’s Hospital,Weifang 261041,China. E-mail: dewdrop 720@163.com |
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Abstract: | AIM: To explore the effect of water extract propolis (WEP) from Taishan on apoptosis of human umbilical vascular endothelial cells (HUVECs) induced in vitro by tumor necrosis factor-α (TNF-α). METHODS: HUVECs were collect by digestion-perfusion and cultivated. TNF-α at concentration of 50 μg/L was administrated to induce the apoptosis of HUVECs. After injury, HUVECs were treated with WEP at concentrations of 50, 100, and 200 mg/L, respectively, for 24 h. Apoptosis was evaluated by terminal-deoxynucleotidyl transferase mediated nick end labeling (TUNEL) and flow cytometry (FCM). RESULTS: Apoptosis index in injured group was significantly higher than that in control group, and decreased significantly after treating with WEP at concentrations of 50, 100, and 200 mg/L, respectively (P<0.01). CONCLUSION: WEP may be useful for protection of vascular endothelial cells by inhibiting apoptosis. |
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Keywords: | Propolis Vascular endothelial cells Apoptosis Tumor necrosis factor |
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