Sodium valproate induces cycle arrest,apoptosis and elevates p21WAF1 mRNA expression in chronic myeloid leukemia cell line K562 |
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| Authors: | ZHANG Xiang-zhong YIN Ai-hua LIU Jian-hua ZHU Xiao-yu HE Min DING Qian CHEN Yun-xian |
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| Institution: | 1.Department of Hematology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China;2.Prenatal Diagnostic Center, Maternal and Children Hospital of Guangdong Province, Guangzhou 510010, China;3.Center for Journal Publishing, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510630, China. E-mail: cyx1228@21cn.com |
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| Abstract: | AIM:To investigate the effects of sodium valproate (VPA) on the cell cycle and apoptosis of chronic myeloid leukemia cell line K562, and to explore the possible mechanisms. METHODS:K562 cells were treated with VPA. Cell cycle and apoptosis were analyzed by flow cytometry. The expression of p21WAF1 mRNA was detected by RT-PCR. RESULTS:After treatment with VPA, cell cycle was arrested obviously at G0/G1 phase ［(82.30±9.41)% vs (40.13±2.12)%, P<0.05］. The apoptotic rate was significantly higher in the cells treated with VPA than that in untreated cells ［(11.47%±0.25%) vs (4.77%±0.40%), P<0.05］. The level of p21WAF1 mRNA was increased ［(1.65±0.91) vs (0.25±0.04), P<0.05］. CONCLUSION:VPA induces elevated expression of p21WAF1 mRNA in K562 cells, resulting in G0/G1 phase arrest and apoptosis in vitro. |
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