Upregulative effect of CGRP on expression of CREB and phospho-CREB protein during focal cerebral ischemia/reperfusion in rat parietal cortex |
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Authors: | ZHANG Zheng-hong XIAO Hong-ju XI Gang-ming QU Peng FANG Xiu-bin |
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Affiliation: | 1.Department of Human Anatomy, 2Department of Neurology, Affiliated Peoples Hospital, 3Department of Internal Medicine, Central Hospital of Dongfeng Corporation, Yunyang Medical College, Shiyan 442000, China;4.Department of Neurobiology, Basic Medical College, China Medical University, Shenyang 110001, China. E-mail: xbfang@mail.cmu.edu.cn |
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Abstract: | AIM: To investigate the effect of calcitonin gene related peptide (CGRP) on the expression of cyclic AMP response element binding protein (CREB) and phosphorylated-CREB in rat parietal cortex during focal cerebral ischemia/reperfusion (I/R).METHODS: Focal cerebral ischemia/reperfusion model was induced by occlusion of the right middle cerebral artery using the intraluminal suture method. The expressions of CREB and phospho-CREB in the parietal cortex in different groups (sham group, focal cerebral ischemia/reperfusion group and CGRP group) were detected with immunohistochemistry and Western blotting, and the positive products were analyzed by image analysis system.RESULTS: There was definite expression of CREB in right parietal cortex in sham group, while it was lesser in I/R group than that in sham group, but it became more in CGRP group than that in I/R group (P<0.05). Phospho-CREB was barely detected in right parietal cortex in sham group and it became more in I/R group than that in sham group. The expression of phospho-CREB increased in CGRP group than that in I/R group of the right parietal cortex (P<0.05).CONCLUSION: CGRP upregulates the expression of CREB and phospho-CREB in the ischemic neurons of the parietal cortex during focal cerebral ischemia/reperfusion and CREB probably involves in the mechanism of protective role of CGRP to ischemic neurons. |
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Keywords: | Brain ischemia Calcitonin gene-related peptide c-AMP response element binding protein Rats Parietal lobe |
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