我国鸡传染性支气管炎病毒地方分离株

根据IBV Beaudette株全基因组序列，借助基因分析软件自行设计合成了3个引物（youl,you2-youM ),引物you1 和you2-在S1基因两则，跨幅为1611bp,引物you2-和youM 在S1基因的3‘端，跨幅为535bp，用引物you1 和you2-对5个IBV地方分离株（HN2、HN4、JX1、SC2和SC4）进行RT－PCR，均成功地扩增出预期大小的目的片段，对RT－PCR的产物用限制性内切酶PstI进行酶切分析，结果酶切产物中得到2条条带，大小分别为560和1050bp，与GeneBank中11株已发表的S1基因分析结果一致，初步鉴定结果显示，已经成功分离得到了IBV－S1基因。

The Amplification of the S1 Gene of Five Chinese Avian Infectious Bronchitis Virus Isolates and Identification

According to the sequence of the genome of the Beaudette strainof IBV, and with a gene analysis program， three primers (you1+、you2- and youM+) were designed and synthesized. The primers you1+ and you2-were located respectively in the 5'-most and the 3'-most end of the S1 gene and approximately cover 1 611 bp and the primer youM+ lying in the middle of the S1 gene. RT-PCR was used to amplify the S1 gene fragment of the five Chinese IBV isolates（HN2，HN4，JX1，SC2 and SC4） using the primers you1+ and you2-. The result indicated that the length of the RT-PCR products was the same as expected. A sequence of 535 bp was amplified by PCR using the primers you2- and you M+ when every RT-PCR product of the five IBV isolates was used for template. The RT-PCR products were digested with the restriction enzyme PstⅠ and two fragments (560 bp and 1 050 bp) were seen, which were the same for the 11 published S1 sequences of IBV strains in Genebank. Therefore , all these results demonstrate that the S1 gene fragments of the five IBV isolates were isolated successfully.