| 培养基组成对金龟子绿僵菌M_(337)菌株稳定性的影响 |
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| 引用本文： | 宋漳,叶斌,叶小真,王安娇,郭文硕. 培养基组成对金龟子绿僵菌M_(337)菌株稳定性的影响[J]. 福建农林大学学报(自然科学版), 2008, 37(2): 135-139 |
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| 作者姓名： | 宋漳 叶斌 叶小真 王安娇 郭文硕 |
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| 作者单位： | 1. 福建农林大学林学院,福建,福州,350002
2. 浙江林学院工程学院,浙江,临安,311300 |
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| 基金项目： | 福建省自然科学基金 , 福建省教育厅资助项目 |
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| 摘 要： | 对金龟子绿僵菌M337菌株进行继代培养,探讨了6种常见培养基、4种碳源和氮源以及6个碳氮组合培养基对菌种稳定性、产孢量、毒力的影响.结果显示,绿僵菌的变异虽然主要受控于菌株自身的遗传特性,但同时也受培养基组成的影响.常见的6种培养基以营养贫乏的CMA和WBA最易使菌种发生变异,PPDA较稳定.动物性氮源较植物性氮源稳定,以麦芽糖和乳糖为碳源的比较稳定.在不同C/N比试验中,供试菌株在C/N比为20/10的培养基上比较稳定.
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| 关 键 词： | 金龟子绿僵菌 稳定性 碳源 氮源 C/N |
| 文章编号： | 1671-5470(2008)02-0135-05 |
| 修稿时间： | 2007-10-25 |
Impact of medium composition on stability of strain M337 of Metarhizium anisopliae |
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| SONG Zhang,YE Bin,YE Xiao-zhen,WANG An-jiao,GUO Wen-shuo. Impact of medium composition on stability of strain M337 of Metarhizium anisopliae[J]. Journal of Fujian Agricultural and Forestry University, 2008, 37(2): 135-139 |
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| Authors: | SONG Zhang YE Bin YE Xiao-zhen WANG An-jiao GUO Wen-shuo |
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| Abstract: | The subcultures of strain M337 of Metarhizium anisopliae were studied in this experiment.Effects of 6 regular media,4 carbon and nitrogen sources and 6 combinations of C and N on strain stability,growth characters and virulence were observed.The results indicated that the variation of M.anisopliae was mainly controlled by its genetic materials,but also under the influence of the composition nature of media.Of the six regular media,strain M337 grew most stably on PPDA,but those nutrition poor ones such as CMA and WBA caused variation very frequently.Among nitrogen sources,those from animal were more stable than those from plant.Meanwhile,strain M337 grew most stably on media containing maltose and lactose as carbon source.The experiment results also showed that strain M337 grew most stably on medium containing C/N ratio at 20/10. |
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| Keywords: | Metarhizium anisopliae stability carbon source nitrogen source C/N |
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