Detection and isolation of small ruminant lentivirus in the amniotic fluid of goats |
| |
| Institution: | 1. Doctorate Student in the Northeast Network in Biotechnology, State University of Ceará, Fortaleza, Ceará, Brazil;2. Embrapa Goats and Sheep, Sobral, Ceará, Brazil;3. Scholarship for Regional Scientific Development of the National Council for Scientific and Technological Development (DCR-CNPq/FUNCAP), Level C, Brasilia, Distrito Federal, Brazil;4. Embrapa Pigs and Poultry, Concórdia, Santa Catarina, Brazil;5. Doctor in Veterinary Sciences, State University of Ceará, Fortaleza, Ceará, Brazil;6. Doctorate Student in Animal Science, Federal University of Piauí, Teresina, Piauí, Brazil;7. Master Degree Student Medical Microbiology, Federal University of Ceará, Fortaleza, Brazil;8. Undergraduate Student in Zootechnics, State University of Acaraú Valley, Sobral, Ceará, Brazil;9. Undergraduate Student in Biological Sciences, State University of Acaraú Valley, Sobral, Ceará, Brazil;10. State University of Ceará, Fortaleza, Ceará, Brazil;1. Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran;2. Department of Clinical Biochemistry, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran;1. Department of Virology, Faculty of Veterinary Medicine, Aydin Adnan Menderes University, Efeler, Aydin, 09016 Turkey;2. Department of Virology, Faculty of Veterinary Medicine, Ankara University, Diskapi, Ankara, 06110, Turkey;1. Centro de Pesquisa em Saúde Animal, Instituto de Pesquisas Veterinárias Desidério Finamor, Departamento de Diagnóstico e Pesquisa Agropecuária, Secretaria da Agricultura, Pecuária e Desenvolvimento Rural, Estrada Do Conde, 6000 - Sans Souci, 92990-000, Eldorado do Sul, Rio Grande do Sul, Brazil;2. Programa de Pós-Graduação em Ciências Veterinárias, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves, 9090 - Agronomia, 91540-000, Porto Alegre, Rio Grande do Sul, Brazil;3. Feevale, Universidade Feevale, Av. Edgar Hoffmeister, 600 - Zona Industrial Norte, Campo Bom, Rio Grande do Sul, Brazil;1. S.C. Sicurezza e Qualità degli Alimenti, Istituto Zooprofilattico Sperimentale Piemonte, Liguria e Valle d’Aosta, Turin, Italy;2. Dipartimento di Malattie Infettive, Istituto Superiore di Sanità, Rome, Italy;3. S.C. Diagnostica Generale, Istituto Zooprofilattico Sperimentale Piemonte, Liguria e Valle d’Aosta, Turin, Italy;4. Laboratorio di Microbiologia e Virologia, Azienda Ospedaliero-Universitaria, Città della Salute e della Scienza di Torino, Turin, Italy;5. Dipartimento di Ispezione degli Alimenti, Istituto Zooprofilattico Sperimentale del Mezzogiorno, Portici (NA), Italy;6. S.C. Epidemiologia ed Analisi del Rischio, Istituto Zooprofilattico Sperimentale Piemonte, Liguria e Valle d’Aosta, Turin, Italy;1. Department of Clinical Sciences, Sanandaj Branch Islamic Azad University, Sanandaj, Iran;2. Department of Pathobiology, Sanandaj Branch Islamic Azad University, Sanandaj, Iran;1. Department of Parasitology and Parasitic Diseases, University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca, Romania;2. Department of Parasitology and Zoology, University of Veterinary Medicine, Budapest, Hungary;3. Section of Virology, Department of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences, Box 7028, 750 07, Uppsala, Sweden;4. SLU Global Bioinformatics Centre, Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, Box 7023, 750 07, Uppsala, Sweden;5. Consejo Nacional de Investigaciones Científicas y Técnicas, Godoy Cruz 2290, 1425, Ciudad Autónoma de Buenos Aires, Argentina;6. Instituto de Agrobiotecnología y Biología Molecular, IABiMo, INTA-CONICET, Calle Las Cabañas y Los Reseros s/n, Casilla de Correo 25, Castelar, 1712, Buenos Aires, Argentina |
| |
| Abstract: | The objective of this study was to verify the presence of small ruminant lentivirus in the amniotic fluid of goats using molecular tests and viral isolation by cocultivation in the amniotic fluid of naturally infected goats. The study analyzed eight goats: seven were small ruminant lentivirus-positive and one was negative. The amniotic fluid was collected from each of the eight animals during cesarean section at 147 days of pregnancy. Cocultivation was undertaken using secondary goat nictitating membrane cell cultures obtained by explant from a small ruminant lentivirus-negative calf followed by trypsinization and sub-cultivation of the cells for 63 days. During this period, five supernatant collections were performed for DNA extraction and subsequent nested polymerase chain reaction. DNA was extracted from the amniotic fluid after 3 h of cellular sedimentation, from which a sample of 600 μL was taken from the sediment and another 600 μL sample from the supernatant. After DNA extraction, nested polymerase chain reaction was performed. Of the eight goats, 62.5 % (05/08) were small ruminant lentivirus-positive, with 43.75 % (07/16) of the total samples positive when considering the two repetitions (supernatant and cell sediment). Moreover, positivity was confirmed by small ruminant lentivirus pro-viral DNA amplification in the cell supernatant throughout the cocultivation period. Small ruminant lentivirus were present in the amniotic fluid samples from the naturally infected goats indicating an intrauterine transmission route. Moreover, this biological fluid can be adopted for the diagnosis of these lentiviruse because it is an important risk factor related to intrauterine transmission. |
| |
| Keywords: | Amniotic sac Goats SRLVs Retrovirus Intrauterine transmission |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
