Antimicrobial resistance in Escherichia coli isolates from frugivorous (Eidolon helvum) and insectivorous (Nycteris hispida) bats in Southeast Nigeria,with detection of CTX-M-15 producing isolates |
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| Affiliation: | 1. Department of Veterinary Public Health and Preventive Medicine, University of Nigeria, Nsukka, Nigeria;2. Area Bioquímica y Biología Molecular, Universidad de La Rioja, Logroño, Spain;3. Microbiology and Antibiotic Resistance Team (MicroART), Department of Veterinary Sciences, University of Trás‐os‐Montes and Alto Douro, Vila Real, Portugal;4. Laboratory of Exploration and Valuation of the Steppe Ecosystem, University of Djelfa, Algeria;5. Institute of Veterinary Sciences, University of Saad Dahlab Blida1, Blida, Algeria;6. Laboratory of Biotechnology Related to Animals Reproduction, Blida, Algeria;7. University of Nigeria Veterinary Antimicrobial Resistance Research Group, Department of Veterinary Pathology and Microbiology, University of Nigeria, Nsukka, Nigeria;1. Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran;2. Department of Medical Parasitology and Mycology, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran;3. Department of Geography, Ardabil Branch, Islamic Azad University, Ardabil, Iran;4. Genetic Engineering and Biotechnology Research Institute University of Sadat City Sadat, Egypt;5. Department of Parasitology and Mycology, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran;6. Infectious Diseases and Tropical Medicine Research Center, Resistant Tuberculosis Institute, Zahedan University of Medical Sciences, Zahedan, Iran;7. Department of Medical Microbiology and Parasitology, College of Health Sciences, Bayero University, PMB 3011, Kano, Nigeria;8. Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran;9. Department of Parasitology and Mycology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran;10. Department of Basic Sciences of Veterinary Medicine, Kazerun Branch, Islamic Azad University, Kazerun, Iran;11. Department of Parasitology, School of Allied Medical Sciences. Ilam University of Medical Sciences, Ilam, Iran;1. Department of Clinical Sciences, Faculty of Veterinary Medicine, Sanandaj Branch Islamic Azad University, Sanandaj, Iran;2. Department of Microbiology, Faculty of Veterinary Medicine, Sanandaj Branch Islamic Azad University, Sanandaj, Iran;1. Imunova Análises Biológicas, Setor de Ciências Biológicas UFPR, Curitiba, PR, 81531-980, Brazil;2. Universidade Federal do Paraná, Setor de Ciências Biológicas UFPR, Curitiba, 81531-980, Brazil;3. Zoetis Indústria de Produtos Veterinários Ltda, São Paulo, SP, 04709-111, Brazil;1. Instituto de Patobiología- UEDD IPVET INTA CONICET, Centro de Investigación en Ciencias Veterinarias y Agronómicas, Instituto Nacional de Tecnología Agropecuaria. Nicolás Repetto y de los Reseros s/n, Buenos Aires, Hurlingham, B1686, Argentina;2. Instituto Nacional de Enfermedades Infecciosas INEI - ANLIS "Dr. Carlos G.Malbran". Av. Vélez Sarsfield 563, Buenos Aires, C1282AFF, Argentina;1. Anhui Province Key Laboratory of Animal Nutritional Regulation and Health, Anhui Science and Technology University, Fengyang, Anhui, China;2. College of Animal Science, Anhui Science and Technology University, Fengyang, China;3. Laboratory of Pork Quality and Safety Control, Ministry of Agriculture and Rural Affairs, Fengyang, Anhui, China |
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| Abstract: | Thirty-five Escherichia coli isolates obtained from the liver, spleen and intestines of 180 frugivorous and insectivorous bats were investigated for antimicrobial resistance phenotypes/genotypes, prevalence of Extended-Spectrum beta-lactamase (ESBL) production, virulence gene detection and molecular typing. Eight (22.9 %) of the isolates were multidrug resistant (MDR). Two isolates were cefotaxime-resistant, ESBL-producers and harbored the blaCTX-M-15 gene; they belonged to ST10184-D and ST2178-B1 lineages. tet(A) gene was detected in all tetracycline-resistant isolates while int1 (n = 8) and blaTEM (n = 7) genes were also found. Thirty-three of the E. coli isolates were assigned to seven phylogenetic groups, with B1 (45.7 %) being predominant. Three isolates were enteropathogenic E. coli (EPEC) pathovars, containing the eae gene (with the variants gamma and iota), and lacking stx1/stx2 genes. Bats in Nigeria are possible reservoirs of potentially pathogenic MDR E. coli isolates which may be important in the ecology of antimicrobial resistance at the human-livestock-wildlife-environment interfaces. The study reinforces the importance of including wildlife in national antimicrobial resistance monitoring programmes. |
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| Keywords: | Bats Antimicrobial resistance ESBL Sequence-type Phylogenetic group EPEC |
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