Investigation of TLR1-9 genes and miR-155 expression in dogs infected with canine distemper |
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| Institution: | 1. Erciyes University, Faculty of Veterinary Medicine, Department of Genetics, Kayseri, Turkey;2. Erciyes University, Faculty of Veterinary Medicine, Department of Internal Medicine, Kayseri, Turkey;3. Ankara University, Faculty of Veterinary Medicine, Department of Biostatistics, Ankara, Turkey;1. Clinic of Medicine, Faculty of Veterinary Science, University of Thessaly, Trikalon Str. 224, 43132, Karditsa, Greece;2. Gastrointestinal Laboratory, Department of Small Animal Clinical Sciences, Texas A&M University, College Station, TX, United States;1. Department of Veterinary Physiology and Biochemistry, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar 125004, Haryana, India;2. Department of Bio-technology, GLA University, Post-Chaumuhan, Dist. Mathura, Uttar Pradesh 281 406, India;3. Department of Veterinary Clinical Complex, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar 125004, Haryana, India;4. Department of Animal Genetics and Breeding, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar 125004, Haryana, India;5. Division of Animal Health, Central institute for Research on Goats (CIRG), Makhdoom, Farah, Mathura, UP 281122, India;1. Department of Microbiology, Mumbai Veterinary College, Parel, Mumbai, 400012, India;2. Department of Animal Genetics Breeding, Mumbai Veterinary College, Parel, Mumbai, 400012, India;3. Department of Animal Biotechnology, Mumbai Veterinary College, Parel, Mumbai, 400012, India;1. Medical Microbiology Laboratory, Department of Microbiology, Center for Excellence in Life Sciences, Bharathidasan University, Tiruchirappalli, 620 024, Tamil Nadu, India;2. Department of Microbiology, College of Veterinary Science, Assam Agricultural University, Guwahati, 781022, Assam, India;3. Department of Parasitology, Tamilnadu Veterinary and Animal Sciences University, Tirunelveli, 600051, India;4. Department of Geography, Bharathidasan University, Tiruchirappalli, 620 024, Tamil Nadu, India;5. Department of Animal Reproduction Gynaecology& Obstetrics (ARGO), College of Veterinary Science, Assam Agricultural University, Guwahati, 781022, Assam, India;1. Department of Biotechnology, Health Sciences Institute, Sağlık Bilimleri University, Istanbul, Turkey;2. Vocational School of Health Sciences, Celal Bayar University, Manisa, Turkey;3. Department of Biology, Faculty of Science, Hacettepe University, Ankara, Turkey;4. Department of Parasitology, Faculty of Veterinary Science, Adnan Menderes University, Aydın, Turkey;5. Veterinary Hospital, Municipality of Karaburun, İzmir, Turkey;6. Department of Parasitology, Faculty of Medicine, Ege University, İzmir, Turkey;1. Dept of Veterinary Clinical Sciences, Faculty of Veterinary Medicine and Animal Science, University of Peradeniya, Sri Lanka;2. Faculty of Agriculture, University of Peradeniya, Sri Lanka |
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| Abstract: | This study aimed to determine the relationship of toll-like receptor (TLR) 1-9 genes and microRNA (miR) -155 expression levels with hematologic parameters in dogs diagnosed with canine distemper. In the study, two groups were used pre-treatment and post-treatment. Infected dogs were diagnosed with canine distemper with the help of a rapid test kit and Real Time-Polymerase Chain Reaction (RT-PCR). Based on the correlation coefficients between the expression levels of the genes examined within the scope of the study and hematologic values, a positive correlation was found between the TLR2 gene and the monocyte (MON) value and between the TLR4 gene and the platelet (PLT) value in the pre-treatment group. A strong positive correlation was identified between TLR3 and TLR9 genes and erythrocyte (RBC) and hemoglobin (HGB) values; between TLR5 gene and RBC, HGB and hematocrit (HCT) values and between TLR9 gene and RBC and HGB values in the post-treatment group, on the other hand, a positive correlation was found between TLR1 gene and MON and neutrophil (GRAN) values; between TLR3 gene and HCT value and between TLR9 gene and MON and HCT values. The study concluded that miR-155 and TLR8 gene were upregulated at a statistically significant level (P < 0.05) Post-treatment in dogs infected with canine distemper and there was a positive correlation between the upregulation of miR-155 and the upregulation of TLR8 in the same period. This result suggests that the upregulated miR-155 expression post-treatment increased TLR8 gene expression. In the light of these findings, it miR-155 may have the potential to be used in clinical practice in the treatment or prognosis of dogs infected with canine distemper. |
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| Keywords: | Dog Gene expression miR-155 TLR |
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