Antibiotic resistance and phylogenetic comparison of human,pet animals and raw milk Staphylococcus aureus isolates |
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| Institution: | 1. Department of Microbiology, College of Science, Agriculture and Modern Technology, Shiraz branch, Islamic Azad University, Shiraz, Iran;2. Department of Pathobiology, Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran;3. Department of Microbiology, Faculty of Basic Sciences, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran;1. Postgraduate Program in Animal Health of the Institute for Veterinary Research Desidério Finamor (IPVDF), Laboratory of Avian Health and Technological Innovation, Department of Agricultural Diagnosis and Research, Secretariat of Agriculture, Livestock and Rural Development of Rio Grande do Sul, Estrada Municipal do Conde, 6000. Sans Souci, Eldorado do Sul, RS, 92990-000, Brazil;2. Postgraduate Program in Animal Science with Emphasis on Bioactive Products, UniversidadeParanaense, Umuarama, PR, Brazil;3. Department of Agricultural Diagnosis and Research, Secretariat of Agriculture, Livestock and Rural Development of Rio Grande do Sul, Rua Gonçalves Dias, 570, Menino Deus, Porto Alegre, RS, 90.130-060, Brazil;1. Laboratório de Imunologia e Doenças Infectocontagiosas. Departamento de Medicina Veterinária. Instituto Federal de Educação, Ciência e Tecnologia da Paraíba, IFPB, Sousa, Paraíba, Brasil;2. Departamento de Morfologia, Universidade Federal da Paraíba, UFPB, João Pessoa, Paraíba, Brasil;1. Departament of Veterinary Medicine, Universidade Federal Rural de Pernambuco, Rua Dom Manuel de Medeiros, sn, Dois Irmãos, Recife 52171-900, Pernambuco, Brazil;2. Department of Forestry Science, Universidade Federal Rural de Pernambuco, Rua Dom Manuel de Medeiros, sn, Dois Irmãos, Recife 52171-900, Pernambuco, Brazil;1. Public Health Institute, University of Porto, Portugal;2. Department of Population Studies, Institute of Biomedical Sciences Abel Salazar, University of Porto, Portugal;3. EPIUnit, Institute of Public Health, ISPUP, University of Porto, Portugal;4. Department of Public Health and Forensic Sciences, and Medical Education, Faculty of Medicine, University of Porto, Porto, Portugal;1. Department of Animal Medicine, Productions and Health, University of Padua, Viale dell’Università, 16-35020, Legnaro, PD, Italy;2. Istituto Zooprofilattico Sperimentale delle Venezie, Viale dell''Università, 10-35020, Legnaro, PD, Italy |
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| Abstract: | The present study was conducted to compare the S. aureus isolates from different sources in the basis of resistance phenotypic and genotypic features and phylogenetic differences. Total of 70 S. aureus isolates (including 25 human, 25 raw milk and 20 pet animal isolates) were subjected to the antimicrobial susceptibility testing, polymerase chain reaction (PCR) detection of the resistance genes and DNA fingerprinting using random amplification of polymorphic DNA–PCR (RAPD-PCR) to survey the variability of the isolates. Among 70 S. aureus, 55 (78.5%) isolates were MRSA. The isolates showed the highest antibiotic resistance to methicillin, ampicillin and penicillin (78.5%) and showed the lowest resistance to ciprofloxacin (12.8%). ErmB and tetM resistance genes were present in all isolates and the vanA gene was not detected in any of the isolates. Thirteen distinct clusters were identified in RAPD-PCR fingerprinting. Statistical analysis showed that the isolates without resistance to antibiotics were significantly in associated with raw milk origin (P < 0.05). According to the results of the study, S. aureus strains with pets and raw milk origin are significant sources of antibiotic-resistant isolates such as MRSA. They are also carriers of resistance genes that can be transmit to human isolates and cause drug resistance in human infections. Identifying the source of these infections is possible with a reliable genotyping method such as RAPD-PCR. |
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| Keywords: | RAPD-PCR MRSA Resistance genes |
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