Non-epidermidis coagulase-negative Staphylococcus isolated from farm animals can inhibit the hemagglutinating activity of Newcastle disease virus and bovine parainfluenza virus type 3 |
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| Institution: | 1. Laboratorio de Brucelosis y Tuberculosis, Departamento de Microbiología e Inmunología, Facultad de Medicina Veterinaria y Zootecnia (FMVZ), Universidad Nacional Autónoma de México (UNAM), Ciudad de México, Mexico;2. Laboratorio de Microbiología Veterinaria, Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas (ENCB), Instituto Politécnico Nacional (IPN), Ciudad de México 11340, Mexico;3. Laboratorio de Virología, Departamento de Microbiología e Inmunología, Facultad de Medicina Veterinaria y Zootecnia (FMVZ), Universidad Nacional Autónoma de México (UNAM), Ciudad de México, Mexico;4. Laboratorio de Bacteriología Médica, Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas (ENCB), Instituto Politécnico Nacional (IPN), Ciudad de México 11340, Mexico;5. Unidad de Investigación en Medicina Experimental, Facultad de Medicina, Universidad Nacional Autónoma de México (UNAM), Ciudad de México 04510, Mexico;6. Área Académica de Nutrición y Toxicología Clínica, Instituto de Ciencias de la Salud, Universidad Autónoma del Estado de Hidalgo, Pachuca Hidalgo, Mexico;7. Laboratorio de Inmunidad Innata, Departamento de Inmunología, Escuela Nacional de Ciencias Biológicas (ENCB), Instituto Politécnico Nacional (IPN), Ciudad de México 11340, Mexico;8. Laboratorio de Inmunomicrobiología, Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas (ENCB), Instituto Politécnico Nacional (IPN), Ciudad de México 11340, Mexico;1. Imunova Análises Biológicas, Setor de Ciências Biológicas UFPR, Curitiba, PR, 81531-980, Brazil;2. Universidade Federal do Paraná, Setor de Ciências Biológicas UFPR, Curitiba, 81531-980, Brazil;3. Zoetis Indústria de Produtos Veterinários Ltda, São Paulo, SP, 04709-111, Brazil;1. Higher Institution Centre of Excellence (HICoE), Tropical Infectious Diseases Research & Education Centre (TIDREC), Universiti Malaya, 50603, Kuala Lumpur, Malaysia;2. Institute for Advanced Studies, Universiti Malaya, 50603, Kuala Lumpur, Malaysia;3. Department of Biology, Faculty of Science, Universiti Putra Malaysia, 43400, Serdang, Selangor Darul Ehsan, Malaysia;4. Institute of Biological Sciences, Faculty of Science, Universiti Malaya, 50603, Kuala Lumpur, Malaysia;1. Department of Pediatric Diseases, Golestan University of Medical Sciences, Gorgan, Iran;2. Student Research Committee, Golestan University of Medical Sciences, Gorgan, Iran;3. Laboratory Sciences Research Center, Golestan University of Medical Sciences, Gorgan, Iran;4. Infectious Diseases Research Center (IDRC), Arak University of Medical Sciences, Arak, Iran;5. Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran;6. Infectious Diseases Research Center, Golestan University of Medical Sciences, Gorgan, Iran;7. Department of Parasitology & Mycology, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran;1. Institut Pasteur du Maroc, Service de Parasitologie et des Maladies Vectorielles, Place Louis Pasteur, Casablanca 20360, Morocco;2. Molecular Genetics and Immunophysiopathology Research Team, Health and Environment Laboratory, Aïn Chock Faculty of Sciences, University of Hassan II Casablanca (UH2C), Casablanca, Morocco;3. Virology Unit, Viral Hepatitis Laboratory, Institut Pasteur du Maroc 1, Place Louis Pasteur, 20360 Casablanca, Morocco;4. Virology Department, Institut Pasteur de Dakar, 36 Avenue Pasteur, Dakar, Senegal;5. UMR1161 Virologie, INRAE, ANSES, Ecole Nationale Vétérinaire d’Alfort, Université Paris-Est, Maisons-Alfort, France;6. Department of Virology, Institut Pasteur, Arboviruses and Insect Vectors Unit, Paris, France;1. Instituto de Patobiología- UEDD IPVET INTA CONICET, Centro de Investigación en Ciencias Veterinarias y Agronómicas, Instituto Nacional de Tecnología Agropecuaria. Nicolás Repetto y de los Reseros s/n, Buenos Aires, Hurlingham, B1686, Argentina;2. Instituto Nacional de Enfermedades Infecciosas INEI - ANLIS \"Dr. Carlos G.Malbran\". Av. Vélez Sarsfield 563, Buenos Aires, C1282AFF, Argentina |
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| Abstract: | The Embp protein of Staphylococcus epidermidis inhibits the hemagglutination of the H1N1 influenza virus and protects birds from a viral respiratory infection. Several species of Coagulase-negative Staphylococcus (CoNS) are present in the respiratory cavity, particularly in nostrils. We hypothesize that non-epidermidis CoNS found in animals can have the same function as observed in S. epidermidis. Thirty Non-epidermidis CoNS isolates were obtained from poultry, sheep, goat, pig, and dairy cow nostrils. Haemagglutination inhibition (HI) activity was assayed in bacteria-free supernatants from non-epidermidis CoNS against Newcastle disease virus (NDV) and bovine parainfluenza virus type 3 (BPIV). In 13 of the 30 strains (43.3 %), bacteria-free supernatants showed HI activity for NDV and BPIV-3. Staphylococcus xylosus supernatants from poultry (one isolate), sheep (two isolates), goat (one isolate), and dairy cow (three isolates) had the highest frequency of HI activity on NDV and BPIV-3, followed by Staphylococcus sp. supernatants from goat (one isolate), dairy cow (two isolates), and finally Staphylococcus equorum, Staphylococcus chromogens and Staphylococcus gallinarum supernatants with single isolation from poultry, pig and poultry, respectively. Nine isolates had the homologous gene to the embp gene of S. epidermidis, and it was associated with HI activity in the studied viruses. By Pulsed‐field gel electrophoresis, S. xylosus isolates showed to be different clones and related to the origin of isolation and HI activity. These results demonstrate that non-epidermidis CoNS supernatants from different animals and origins have the ability of HI on NDV and BPIV-3, indicating that not only S. epidermidis has the same function. |
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| Keywords: | NDV BPI-3 Hemagglutination inhibition Supernatant |
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