Phylogenetic analysis of kinetoplast DNA: kDNA of Leishmania tropica in Thi-Qar province,Iraq |
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| Institution: | 1. Department of Medical Laboratory Techniques, Nasiriyah Technical Institute, Southern Technical University, Nasiriyah 64001, Iraq;2. Department of Biology, College of Education for Pure Sciences, University of Thi-Qar, Nasiriyah 64001, Iraq;1. Infectious Diseases Research Center, Health Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran;2. Liver and Digestive Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran;3. Department of Medical Laboratory Sciences, School of Paramedical, Kermanshah University of Medical Sciences, Kermanshah, Iran;4. Department of Laboratory Sciences, Maragheh University of Medical Sciences, Maragheh, Iran;5. Department of Heath, Maragheh University of Medical Sciences, Maragheh, Iran;1. Department of Clinical Sciences, Sanandaj Branch Islamic Azad University, Sanandaj, Iran;2. Department of Pathobiology, Sanandaj Branch Islamic Azad University, Sanandaj, Iran;1. Doctorate Student in the Northeast Network in Biotechnology, State University of Ceará, Fortaleza, Ceará, Brazil;2. Embrapa Goats and Sheep, Sobral, Ceará, Brazil;3. Scholarship for Regional Scientific Development of the National Council for Scientific and Technological Development (DCR-CNPq/FUNCAP), Level C, Brasilia, Distrito Federal, Brazil;4. Embrapa Pigs and Poultry, Concórdia, Santa Catarina, Brazil;5. Doctor in Veterinary Sciences, State University of Ceará, Fortaleza, Ceará, Brazil;6. Doctorate Student in Animal Science, Federal University of Piauí, Teresina, Piauí, Brazil;7. Master Degree Student Medical Microbiology, Federal University of Ceará, Fortaleza, Brazil;8. Undergraduate Student in Zootechnics, State University of Acaraú Valley, Sobral, Ceará, Brazil;9. Undergraduate Student in Biological Sciences, State University of Acaraú Valley, Sobral, Ceará, Brazil;10. State University of Ceará, Fortaleza, Ceará, Brazil;1. Scientific and Technical Research Centre for Arid Areas (CRSTRA), Biophysical Station, PB 30240, Nezla, Touggourt, Algeria;2. Animal Science Department, Iowa State University, Ames, IA, 50011, USA;3. Higher National Veterinary School, PB 161 Rue Issad Abbes, Oued Smar, Algiers, Algeria;4. Institute of Veterinary Sciences, LBRA, University of Blida 1, PB 270, Soumaa, Blida, Algeria;5. UMR SELMET, CIRAD-ES, Montpellier, France |
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| Abstract: | IntroductionCutaneous leishmaniasis (CL) is one of wobbling endemic disease in Iraq, that cause intracellular obligate protistan parasite returned to the genusLeishmania. This study is aimed to identify epidemiology of CL, detect the prevalence of Leishmania tropica and find the phylogenetic relationship.MethodologyThe current study was conducted in the main hospitals of Thi-Qar province-south of Iraq for period from November 2018 to October 2019. Nested-PCR was used to amplify kinetoplast minicircle fragments DNA.ResultsIt was recorded 247 clinical cases with CL, the infections of males were higher than females, while infection rate appeared gradual reduction with age progress. Furthermore, the most CL infections were as single lesions and occurred in December. The infections of upper limbs were high when compared with other body regions. The molecular diagnosis showed L. tropica was more frequently. DNA sequences of kDNA gene of L. tropica showed confirmative genetic detection of local isolates using NCBI-Blast data and phylogenetic tree analysis after comparison with global recorded isolates. The local L. tropica isolates showed genetically closed related to NCBI-Blast L. tropica with accession number AB678350.1. Generally, the analysis of kDNA nitrogen bases sequences showed that all of samples were consistent with those recorded at the NCBI.ConclusionThe kDNA minicircle sequences analysis results showed mismatching of the local isolates decrease whenever approached from the Iranian border. In addition, genetic heterogeneity diagnosis is important for detection of therapy, control and epidemiological studies. |
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| Keywords: | Cutaneous leishmaniasis Phylogenetic tree kDNA sequences Kinetoplast minicircles DNA Dermal lesion |
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