The molecular and serological investigation of Feline immunodeficiency virus and Feline leukemia virus in stray cats of Western Turkey |
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| Institution: | 1. Department of Virology, Tekirdag Namik Kemal University, Faculty of Veterinary Medicine, Tekirdag 59030, Turkey;2. Department of Molecular Biology, Ege University Faculty of Sciences, Bornova, Izmir, 35100, Turkey;3. Department of Parasitology, Ege University Medical School, Bornova, Izmir, 35100, Turkey;4. Odemiş Training Collage, Ege University, Odemiş, İzmir, 35400, Turkey;5. The Protection and Development Union of Izmir Bird Paradise, Izmir, Turkey;6. Izmir Atatürk Training and Research Hospital, Department of Microbiology, Yeşilyurt, Izmir, Turkey;7. Department of Medical Microbiology, Hamidiye Faculty of Medicine, University of Health Sciences, İstanbul, Turkey;8. Department of Parasitology, Tekirdag Namik Kemal University, Faculty of Veterinary Medicine, Tekirdag 59030, Turkey;1. Department of Clinical Sciences, Sanandaj Branch Islamic Azad University, Sanandaj, Iran;2. Department of Pathobiology, Sanandaj Branch Islamic Azad University, Sanandaj, Iran;1. Department of Parasitology, Ege University, Faculty of Medicine, Izmir, Turkey;2. Department of Internal Medicine, Faculty of Veterinary Medicine, Uludağ University Institute of Health Sciences, Görükle Campus, Nilüfer, Bursa, Turkey;3. İzmir Natural Life Park Branch Directorate, Municipality of İzmir, Sasalı, Çiğli, İzmir, Turkey;4. The Protection and Development Union of İzmir Bird Paradise, Sasalı, Çiğli, İzmir, Turkey;5. Department of Biology, Molecular Biology Section, Ege University Faculty of Science, Bornova, İzmir, Turkey;1. Laboratory of Molecular Immunology and Infectious Disease, The United Graduate School of Veterinary Science, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8515, Japan;2. Laboratory of Molecular Immunology and Infectious Disease, Joint Faculty of Veterinary Medicine, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8515, Japan;3. Department of Molecular Life Science, Tokai University School of Medicine, 143 Shimokasuya, Isehara, Kanagawa 259-1193, Japan;4. Laboratory of Small Animal Internal Medicine, Joint Faculty of Veterinary Medicine, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan;5. Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan;1. Clinic for Gastroenterology, Hepatology, and Infectiology, Medical Faculty, Heinrich-Heine-University Düsseldorf, 40225 Düsseldorf, Germany;2. Laboratório de Virologia, Departamento de Microbiologia, Imunologia e Parasitologia, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil;3. Max von Pettenkofer Institute & Gene Center, Virology, National Reference Center for Retroviruses, Faculty of Medicine, LMU München, Munich, Germany;4. InBIO – Research Network in Biodiversity and Evolutionary Biology, CIBIO, Campus de Vairão, Universidade do Porto, Vairão, Portugal;5. Departamento de Biologia, Faculdade de Ciências, Universidade do Porto, Porto, Portugal;6. Department of Microbiology, Immunology, and Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO, USA;1. Sydney School of Veterinary Science, The University of Sydney, Sydney, NSW 2006, Australia;2. Centre for Veterinary Education, The University of Sydney, Sydney, NSW 2006, Australia |
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| Abstract: | This study aimed to investigate the Feline immunodeficiency virus (FIV) / Feline leukemia virus (FeLV) infection prevalence among looking healthy stray cats in Western Turkey by serologic and molecular-based tests. A total of 1008 blood samples from the stray cats were used in this study. All samples were tested for FIV antibodies / proviral DNA and FeLV antibodies / antigens / proviral DNA. The genetic characterization and phylogenetic analysis of FeLV and FIV were carried out in this study. These cats also tested for Leishmaniasis and Toxoplasmosis previously. FIV Ab and proviral DNA detected in 25.2 % and 25.5 % of samples, respectively. FeLV Ab, Ag, proviral DNA positivity was in 45.2 %, in 3.3 %, in 69.7 %, respectively. The molecular detection and phylogenetic analysis of the current FeLV pol gene and FIV gag gene performed. The molecular characterization for the pol gene of FeLV (enFeLV and exFeLV) among Turkey's cat population was reported for the first time. The exFeLV pol sequences closer to the FeLV-A genotype, and the enFeLV pol sequences overlapped with other enFeLV. The current FIV gag sequences were clustered within the subtypes A, B, and C. The findings revealed FeLV subtype A and FIV subtype-A, subtype-B, subtype-C circulate among Turkish stray cats. Single and multiple co-infection positivity was found higher compared to previous reports. |
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| Keywords: | FIV FeLV Serological survey Molecular survey Stray cats Turkey |
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