两种染色法测定禽源性大肠杆菌分离株的外膜蛋白型

对江苏地区２５个禽源性大肠杆菌Ｏ＿１、Ｏ＿２和Ｏ＿（７８）分离株的外膜蛋白型（ＯＭＰ型）进行测定。用Ｎ－十二烷酰肌氨酸（Ｓａｒｋｏｓｙｌ）提取其外膜蛋白，经ＳＤＳ－ＰＡＧＥ电泳后，分别以考马斯亮蓝染色法和银染法测定其外膜蛋白型。８个Ｏ＿１分离株、９个Ｏ＿２分离株分属２个ＯＭＰ型，其中的１个为二者所共有，而８个Ｏ＿（７８）分离株的ＯＭＰ型亦与该型用同；两种染色法对ＯＭＰ型的测定结果一致。与银染法相比，考马斯亮蓝染色法具有简便和背景清晰的优点，因此，考马斯亮蓝染色法是禽源性大肠杆菌分离株ＯＭＰ型测定的更为理想的染色方法

OUTER MEMBRANE PROTEIN (OMP) PATTERNS OF ESCHERICHIA COLI ISOLATES OF CHICKEN ORIGIN VISULIZED WITH COOMASSIE BRILLIANT BLUE STAINING AND SILVER STAINING

OMP patterns of twenty-five isolates of Escherichia coli serotype O_1, O_2 and O_(78) of chicken origin from Jiang-su area were determined.The outer membrane proteins of these isolates were isolated with N-laurovlsarcosine and analyzedby SDS-PAGE. Major outer membrane proteins bands contributed for OMP patterns were visualized with Ccomassie bril-liant blue staining and silver staining. 2 OMP patterns were divided in eight of O_1 isolates and nine O_2 isolates respectivly.Among these OMP patterns 1 OMP pattern was shared by these,2 different O serotypes isolates, and eight of O_(78) also sharedthis OMP pattern. The same OMP patterns were represented for each of isolates with both of staining procedues. Comparedwith silver staining, Coomassie brilliant blue staining had the advantage of simpless and clear background. These results il-lustrated that Coomasie brilliant blue staining would be better than silver staining for the analysing of OMP patterns of Es-cherichia coli isolates of chicken origin.