江苏山羊品种细胞色素b基因(Cyt b)变异特点及其系统地位分析

测定了江苏山羊品种的细胞色素b基因全序列1 140 bp,并引用10个山羊品种(群体)细胞色素b基因全序列进行比较,分析碱基组成和变异情况以及核苷酸序列差异.以绵羊为外群,分别采用邻近法、最大简约法和最大似然法构建分子系统树,得到的拓扑结构一致,初步提示了江苏山羊品种的系统进化关系:长江三角洲白山羊和黄淮山羊的亲缘关系较近,可能来自同一个母系;它们与日本山羊可能在较早的世代具有共同的祖先.本研究结果也支持将东亚、南亚和东南亚固有山羊群体划分为"东亚"和"南亚"两大亲缘系统的观点.

Variance Characteristics of Mitocondrial Cytochrome b Gene (Cyt b) and Phylogeny of Jiangsu Goat Breeds

To analyse the base composition and variance and difference in nucleotide,complete sequences of mt DNA cytochrome b gene(1 140 bp) in Jiangsu goat breeds were determined and compared with cytochrome b gene in other 10 goat breeds (populations).The molecular phylogenetic trees constructed by means of neighbor joining,Maximum parsimony,and maximum likeness according to the analysis supported the same topology,which revealed the phylogenetic relationship between Jiangsu goat breeds.Yangtse River Delta White goat had a very close relationship to Huanghuai goat.They might origin from one maternal line.They might also share the same ancestor with Japanese goats.The results were in favor of the opinion that the native goat populations to the east and south of Central Asia could be classified into two genetic groups,'East Asia' and 'South Asia'.