| 诱变苜蓿愈伤组织抗寒性研究 |
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| 引用本文： | 李波,贾秀峰,高美玲,贲文伟. 诱变苜蓿愈伤组织抗寒性研究[J]. 草地学报, 2004, 12(2): 95-97. DOI: 10.11733/j.issn.1007-0435.2004.02.004 |
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| 作者姓名： | 李波 贾秀峰 高美玲 贲文伟 |
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| 作者单位： | 齐齐哈尔大学生命科学与工程学院, 黑龙江, 齐齐哈尔, 161006 |
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| 基金项目： | 黑龙江省教育厅科学技术研究项目 |
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| 摘 要： | 以3个苜蓿品种幼茎诱导产生的愈伤组织为材料,用叠氮化钠进行诱变处理,经-7℃低温筛选后,测定存活愈伤组织的可溶性糖含量、过氧化物酶活性以及脯氨酸含量等生理生化指标。结果表明,草原2号、准格尔、龙牧801三个苜蓿品种诱变处理后愈伤组织的抗寒性明显高于对照。
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| 关 键 词： | 生物技术 苜蓿品种 抗寒性 NaN3 诱变处理 愈伤组织 |
| 文章编号： | 1007-0435(2004)02-0095-03 |
| 收稿时间： | 2003-08-14 |
| 修稿时间： | 2003-08-14 |
A Research on the Cold-Resistance of Induced Alfalfa Calluses |
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| LI Bo,JIA Xiu-feng,GAO Mei-ling,BEN Wen-wei. A Research on the Cold-Resistance of Induced Alfalfa Calluses[J]. Acta Agrestia Sinica, 2004, 12(2): 95-97. DOI: 10.11733/j.issn.1007-0435.2004.02.004 |
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| Authors: | LI Bo JIA Xiu-feng GAO Mei-ling BEN Wen-wei |
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| Affiliation: | College of Life Science and Engineering, Qiqihar University, Qiqihar, Heilongjiang Province 161006, China |
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| Abstract: | The stems from induced calluses of three alfalfa varieties-Caoyuan No.2, Neimeng Zhungeer, and Longmu 801--were mutagenized with NaN3,and screened from mutants after culturing in a temperature of (-7℃). The living calluses were then measured for their physiological and biochemical characters,such as soluble sugar content, peroxidase activity,and proline content, ect. The result shows that the three alfalfa varieties boast high cold-resistant capacity than the control. |
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| Keywords: | Biological technique Alfalfa varieties Cold-resistance NaN_3 Mutagenize Callus |
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