Somaclonal variation in cell suspension culture-derived regenerants of Cymbopogon martinii (Roxb.) Wats var. motia |
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Authors: | J. Patnaik S. Sahoo B. K. Debata |
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Affiliation: | Aromatic and Medicinal Plants Division, Regional Research Laboratory, Bhubaneswar-751013, India |
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Abstract: | Plants regenerated from cell suspension cultures of palmarosa grass, Cymbopogon martinii (Roxb.) Wats, were analysed for somaclonal variation in five clonal generations. A wide range of variation in important quantitative traits, e.g. plant yield, height, tiller number, oil content and qualitative changes in essential oil constituents—geraniol, geranyl acetate, geranyl formate and linalool—were observed among the 120 somaclones screened. Eight somaclones were selected on the basis of high herb and oil yield over the donor line and high geraniol content in the oil. Based on performance in the field trials, three superior lines were selected, and maintained for five clonal generations. The superior lines exhibited a reasonable degree of stability in the traits selected. |
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Keywords: | Cymbopogon martinii clonal generation somaclonal variation |
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