白菜病程相关蛋白1基因cDNA全长的克隆与分析

 以水杨酸处理后的‘苏州青’白菜为材料, 通过RT2PCR和RACE技术, 获得了白菜病程相关蛋白PR-1基因的cDNA全长序列。该基因与甘蓝型油菜PR-1基因氨基酸序列的同源性为91% , 与拟南芥的同源性为78% , 与其它植物的同源性为57%～60%。Southern杂交表明该基因在白菜基因组中的拷贝数至少为2个。半定量RT2PCR分析表明, 2 mmol/L的水杨酸处理后12 h, 该基因的表达量达到高峰。

Cloning and Characterization of a Full-length cDNA of Pathogenesis-related Protein 1 in Brassica rapa ssp. chinensis

In this study, according to the consensus domain of the Brassica napus pathogenesis-related protein PR-1 gene(accession number U21849),using RT-PCR and RACE technology, a full-length cDNA of pathogenesis-related protein 1 named BcPR-1 was obtained from Brassica rapa ssp.chinensis cultivar Suzhouqing, which displayed resistance to downy mildew. Sequence analysis indicated that cloned BcPR-1 gene consisted of 646 nucleotides (nt) containing 161 amino acids. Further comparison to B. napus PR-1 gene and Arabidopsis thaliana PR-1 gene showed that its identity was 91% and 78%, respectively. Its similarity to other PR-1 genes was 57% to 60%. Southern-blot analysis indicated that there were at least two copies of BcPR-1 gene in B. rapa ssp.chinensis genome. Semi-quantitative RT-PCR analysis revealed that expression of BcPR-1 gene was induced by SA treatment in Suzhouqing, and the corresponding mRNA was accumulated most abundantly 12 h after treatment upon 2 mmol/L SA.