Biochemical,physiological, and molecular characterization of Dickeya dianthicola (formerly named Erwinia chrysanthemi) causing potato blackleg disease in Japan |
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| Authors: | Taketo Fujimoto Shinji Yasuoka Yoshiyuki Aono Takato Nakayama Takehiro Ohki Mitsuru Sayama Tetsuo Maoka |
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| Affiliation: | 1.Hokkaido Agricultural Research Center, National Agriculture and Food Research Organization (HARC/NARO),Sapporo,Japan;2.Tokachi Agricultural Experiment Station,Hokkaido Research Organization,Memuro,Japan;3.Hokkaido-Chuo Station, Center for Seeds and Seedlings, NARO,Kitahiroshima,Japan |
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| Abstract: | The taxonomic assignment of Japanese potato blackleg isolates of Dickeya spp. has not been confirmed after the changes in their former name, Erwinia chrysanthemi. Therefore, we investigated and identified 23 representative isolates of Dickeya spp. from symptomatic stems of potatoes in Japan, with biochemical tests and phylogenetic sequence analysis using recA, dnaX, rpoD, gyrB, and 16S rDNA sequences. Results of our biochemical tests showed that all isolates can be assigned to phenon 5 and biovar 1, which are associated with D. dianthicola. Based on the recA, dnaX, rpoD, gyrB, and 16S rDNA sequences, all isolates are in the same clade with D. dianthicola and were clearly distinguished from D. chrysanthemi, D. dadantii, D. dadantii subsp. dieffenbachiae, D. solani, D. zeae, and D. paradisiaca. Therefore, we conclude that Dickeya spp. isolated from potatoes with blackleg symptoms in Japan are D. dianthicola. |
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