绵羊卵巢卵泡颗粒细胞体外培养中促卵泡素和胰岛素浓度优化研究

为了进一步探讨在无血清体外培养条件下,促卵泡素(FSH)和胰岛素对绵羊卵巢颗粒细胞的影响,以优化颗粒细胞体外培养体系。试验设计6个FSH浓度梯度和3个胰岛素浓度梯度。体外培养7d后,检测培养体系中颗粒细胞数和雌激素浓度。与对照组相比,培养液中添加FSH与胰岛素可显著提高颗粒细胞的增殖和雌激素的分泌(P<0.01);且随着FSH浓度的增加,颗粒细胞数和雌激素分泌量显著增加,当FSH浓度为5.0μg·L-1时,雌激素分泌量最高;随着胰岛素浓度增加,颗粒细胞数和雌激素分泌量显著增加,当胰岛素浓度为10μg·L-1时,颗粒细胞数和雌激素分泌量最高。

Concentration Optimization of Follicle-Stimulating Hormone and Insulin on in Vitro Cultured Ovarian Follicular Granulosa Cells in Sheep

To study more effects of follicle-stimulating hormone(FSH) and insulin on sheep ovarian granulosa cells in serum-free culture in vitro and obtain optimal culture system,we used six different concentrations of FSH and three of insulin.Cell number and E2 concentration were calculated after 7 days.The results indicated that groups treated with FSH or insulin showed a notable increment in stimulating granulosa cell proliferation and estradiol production than control groups(P<0.01).Granulosa cells and estradiol production were more,accompanying the higher concentrations of two additives.E2 concentration was the highest,when FSH was 5 μg·L-1.The most granulosa cells and the highest E2 concentration occurred when insulin was 10 μg·L-1.We obtained the best culture concentrations of FSH and insulin in vitro.