葡萄着色期果皮蛋白质组分析

为了研究葡萄不同着色期果皮中蛋白质表达特征,以着色初期、中期和后期等3个着色时期的葡萄果皮为研究对象,运用2-DE、MALDI-TOF/TOF-MS质谱技术以及生物信息学方法对差异蛋白进行分析,结果表明:(1)双向凝胶电泳显示,果皮着色3个时期有1050个高度重复的蛋白点,差异显著的蛋白点有162个,其中108个蛋白得到鉴定,有87个蛋白映射到葡萄蛋白质组数据库中;3个时期均表达的差异蛋白有20个,且随着果皮颜色加深,差异蛋白数量呈上升趋势。(2)GO分析显示,ATP合成酶β亚基(ATPβ)极显著富集于磷酸核糖代谢过程,对着色初期果皮生理代谢的能量需求具有重要意义。(3)KEGG分析表明,碳代谢、生物固碳、磷酸戊糖、氨基酸生物合成等代谢通路在果皮着色的不同时期显著富集。(4)qRT-PCR分析显示,S–腺苷甲硫氨酸合成酶基因(VvMETK4)在果皮着色后期表达量最高。

Proteome Analysis of Pericarp During Grape Coloring Period

In order to study protein expression characteristics of grape pericarp in different coloring periods，early，middle and later stages of pericarp coloring were studied. 2-DE（Two-dimensional gel electrophoresis）and MALDI-TOF/TOF-MS（mass spectrometry technology）was used to analyze the differences of protein expression in pericarp at different coloring stages. (1) 2-DE revealed that there were 1 050 highly repeated protein spots during 3 periods of pericarp coloring，and 108 of 162 differential- expressed proteins were identified，87 proteins were mapped to the grape database. Twenty proteins differentially expressed during 3 periods of pericarp coloring，and the number of differential-expressed proteins was increasing with the deepening process of pericarp color. (2) GO enrichment analysis showed ATPβ（ATPase β subunit）was highly enriched in the ribose phosphate metabolic process，which was of great significance to the energy requirement of physiological metabolism in the early stage of coloring. (3) KEGG analysis showed that many KEGG pathways，such as carbon metabolism，photosynthetic carbon fixation，pentose phosphate，amino acid biosynthesis，metabolic pathways etc，were significantly enriched at different stages of pericarp coloration. (4) qRT-PCR analysis showed that the S-adenosylmethionine synthetase 4 gene（VvMETK4）had the highest expression level in the later stage of pericarp coloring.