佛手种质资源遗传多样性的ISSR 分析

 收集佛手（Citrus medica L. var. sarcodactylis Swingle）13 份、枸橙（C. medica var. yunnanensis Ding）1 份及香椽（C. medica L.）6 份，共20 份种质资源，其中14 份成功引种在佛手资源圃。在对其中 14 份资源生物学特性进行描述的基础上，采用ISSR 分子标记技术对20 份样品进行遗传多样性分析。筛 选出的11 条引物共检测到102 条DNA 条带，其中58 条为多态性条带，多态性条带比率为56.9%。各样 品之间的遗传相似性系数在0.608 ~ 0.990 之间。聚类分析结果表明，当遗传系数为0.798 时，可将供试材 料分为枸橙、香橼和佛手3 个类群，新发现的3 个芽变材料是佛手中一个新的类群。

Genetic Diversity Analysis of Fingered Citron Germplasm by ISSR

Thirteen fingered citron accessions，1 citrate acid orange and 6 citron accessions were collected and fourteen genotypes were introduced and survived in the germplasm resources garden. Based on the biological traits，the genetic diversities of 20 samples were evaluated by ISSR markers. A total of 102 DNA bands were amplified from the 11 polymorphic primers，which were screened from 100 ISSR primers. Among those obtained bands，58 were polymorphic ones，accounting for 56.9% polymorphism. The genetic similarity coefficient among the accessions ranged from 0.608 to 0.990. At the point of the similarity coefficient 0.798，all the accessions might be separated into three groups，fingered citron，citrate acid orange and citron，meanwhile three pieces of Yabian mutants belonged to a new group of fingered citron.