生物炭对砂土水肥保持及苹果生长的影响研究

为解决砂土易漏水漏肥的问题,以富士/平邑甜茶为试材,探讨了生物炭在砂土苹果园中的应用效果。结果表明,生物炭与化肥配合穴施增加了优质果率,提高了单果质量与单株产量,分别达到70.21%、261.92 g和59.62 kg,较传统单独穴施化肥的对照分别增加了43.40%、15.21%和36.06%;可溶性固形物和维生素C含量较对照提高了7.10%和30.30%,果实品质得到改善;土壤速效氮和速效钾含量分别达153.58和142.2 mg·kg~(-1),较对照提升15.69%和33.27%;在降水后第3天生物炭施用土层土壤含水量较上、下土层分别高36.65%和27.92%,1个月后仍然分别高56.65%和45.79%。结果显示,生物炭与化肥配合穴施的方法在砂土苹果园中对其肥水的保持及苹果产量和品质的提高均有显著效果。

Effect of Biochar on Water and Nutrient Conservation and Apple Growth in Sandy Soil

AIM:To investigate the effect of inhibiting interferon-γ signaling pathway on the immune regulation of rat bone marrow mesenchymal stem cells (BMMSCs) by silencing the expression of interferon-γ receptor (IFN-γR) gene. METHODS:According to the rat IFN-γR gene sequence, lentivirus carrying INF-γR shRNA (shIFNγR-LV) was constructed and transfected into the rat BMMSCs to silence the expression of IFN-γR gene. The lymphocyte proliferation ability and the concentrations of inflammatory factors in control group, BMMSCs negative control (BMMSC-NC) group and BMMSC-shIFNγR group were detected by mixed lymphocyte culture experiments. RESULTS:The constructed shIFNγR-LV significantly decreased the mRNA expression of IFN-γR in the rat BMMSCs. Mixed lymphocyte culture experiments showed that the inhibition of lymphocyte viability was weakened in BMMSC-shIFNγR group compared with BMMSC-NC group (P<0.05). In the mixed lymphocyte culture system, the concentration of tumor necrosis factor-α in BMMSC-NC group was lower than that in BMMSC-shIFNγR group, and the concentration of interleukin-10 was higher than that in BMMSC-shIFNγR group (P<0.05). CONCLUSION:RNA interference technique effectively silences the expression of IFN-γR gene in the rat BMMSCs and significantly reduces the immune function of BMMSCs.