新型试管花卉姬松高效再生体系的研究

 以新型试管花卉多肉类植物姬松（Crassula clavata N. E. Brown）为材料进行高效再生体系的研究。结果表明：以姬松茎尖为外植体的初代培养中，MS 培养基添加IBA 并未诱导出根系生成，而是直接诱导出丛生芽，在MS + IBA 0.3 mg · L-1 培养基上，由叶片表面诱导出的丛生芽质量最佳；在继代培养中，最佳培养基为MS + NAA 0.1 mg · L-1 + 6-BA 0.5 mg · L-1，诱导产生大量胚性愈伤组织，且表面有芽体分化；在彩色培养中，在1/2MS 中添加0.25 mg · L-1 的食用色素亮蓝，观赏效果佳且对植株生长无毒害。

A Highly Efficient in Vitro Regeneration Protocol for Crassula clavata as Novel Tube-flower

Tube-flower is a novel form of in vitro plant products. A highly efficient in vitroregeneration system was established for the production of succulent plant Crassula clavata N. E. Brown.In primary culture，adventitious buds instead of adventitious roots were differentiated from the shootexplants on MS medium supplemented with different concentrations of IBA；The highest multiplicationrate and high-quality adventitious buds were obtained on the optimal medium of MS containing 0.3mg · L-1 IBA. In subculture，the medium of MS containing 0.1 mg · L-1 NAA and 0.5 mg · L-1 6-BA wasscreened out for large amounts of embryogenic callus could be significantly induced and sprouts could befurther differentiated on the callus surface. In culture for colorful decorative products，0.25 mg · L-1 ediblecolourant to half strength MS medium was feasible to give pleasant visual effect without harmful or toxiceffect on the in vitro plants.